THE INTRON REQUIREMENT FOR IMMUNOGLOBULIN GENE-EXPRESSION IS DEPENDENT UPON THE PROMOTER

被引：71

作者：

NEUBERGER, MS ^{[1
]}

WILLIAMS, GT ^{[1
]}

机构：

[1] MRC, MOLEC BIOL LAB, HILLS RD, CAMBRIDGE CB2 2QH, ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1988年 / 16卷 / 14期

关键词：

D O I：

10.1093/nar/16.14.6713

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transfection assays were used to assess the need for an intron in order to obtain expression of cytoplasmic immunoglobulin .mu. mRnA. An intron is required when transcription is driven by an immunoglobulin promoter/enhancer combination, although this requirement is not specific for a particular intron. However, this need for an intron is dependent upon the promoter used. Whilst an intron is required in the case of immunoglobulin or .beta.-globin promoters, it is not in the case of cytomegalovirus or heat-shock promoters. The data point to a connection between the promoter and RNA processing or export.

引用

页码：6713 / 6724

页数：12

共 33 条

[1] GENE GATING - A HYPOTHESIS [J].

BLOBEL, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (24) :8527-8529

[2] A VERY STRONG ENHANCER IS LOCATED UPSTREAM OF AN IMMEDIATE EARLY GENE OF HUMAN CYTOMEGALO-VIRUS [J].

BOSHART, M ;

WEBER, F ;

JAHN, G ;

DORSCHHASLER, K ;

FLECKENSTEIN, B ;

SCHAFFNER, W .

CELL, 1985, 41 (02) :521-530

[3] HEAVY-CHAIN VARIABLE REGION CONTRIBUTION TO THE NPB FAMILY OF ANTIBODIES - SOMATIC MUTATION EVIDENT IN A GAMMA-2A VARIABLE REGION [J].

BOTHWELL, ALM ;

PASKIND, M ;

RETH, M ;

IMANISHIKARI, T ;

RAJEWSKY, K ;

BALTIMORE, D .

CELL, 1981, 24 (03) :625-637

[4] INTRONS INCREASE GENE-EXPRESSION IN CULTURED MAIZE CELLS [J].

CALLIS, J ;

FROMM, M ;

WALBOT, V .

GENES & DEVELOPMENT, 1987, 1 (10) :1183-1200

[5] SYNTHESIS OF AN UNSPLICED CYTOPLASMIC MESSAGE BY AN ADENOVIRUS 5 DELETION MUTANT [J].

CARLOCK, L ;

JONES, NC .

NATURE, 1981, 294 (5841) :572-574

[6] POLYMERIC IMMUNOGLOBULIN-M IS SECRETED BY TRANSFECTANTS OF NONLYMPHOID CELLS IN THE ABSENCE OF IMMUNOGLOBULIN-J CHAIN [J].

CATTANEO, A ;

NEUBERGER, MS .

EMBO JOURNAL, 1987, 6 (09) :2753-2758

[7] GENE-EXPRESSION FROM BOTH INTRONLESS AND INTRON-CONTAINING ROUS-SARCOMA VIRUS CLONES IS SPECIFICALLY INHIBITED BY ANTI-SENSE RNA [J].

CHANG, LJ ;

STOLTZFUS, CM .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (09) :2341-2348

[8] EXPRESSION OF A BEAN STORAGE PROTEIN PHASEOLIN MINIGENE IN FOREIGN PLANT-TISSUES [J].

CHEE, PP ;

KLASSY, RC ;

SLIGHTOM, JL .

GENE, 1986, 41 (01) :47-57

[9] ENHANCEMENT OF MESSENGER-RNA NUCLEAR TRANSPORT BY PROMOTER ELEMENTS [J].

DELAPENA, P ;

ZASLOFF, M .

CELL, 1987, 50 (04) :613-619

[10] EXPRESSION OF ABBREVIATED MOUSE DIHYDROFOLATE-REDUCTASE GENES IN CULTURED HAMSTER-CELLS [J].

GASSER, CS ;

SIMONSEN, CC ;

SCHILLING, JW ;

SCHIMKE, RT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (21) :6522-6526

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