AFFINITY CHROMATOGRAPHY OF ENDOGLUCANASE OF TRICHODERMA-VIRIDE BY CONCANAVALIN AAGAROSE

Endoglucanase (Cx cellulase) and cellobiase are often cross‐contaminated in separation procedures by ion‐exchange chromatography such as DEAE‐cellulose. By using concanavalin A (Con A)–agarose chromatography, Cx cellulase and cellobiase from Trichoderma viride can be separated. Cx cellulase showed affinity toward Con A, indicating a glycoprotein containing α‐D‐mannopyransyl and α‐D‐glucopyranosyl end groups or internal 2‐O‐D‐mannopyranosyl residues in sugar moieties. This method provides a way to estimate the quantities of Cx enzyme produced by T. viride and possibly by other organisms. Copyright © 1979 John Wiley & Sons, Inc.