STIMULATION AND INHIBITION BY PUROMYCIN OF CELL-FREE AMINO ACID INCORPORATION

被引：7
作者：
STENESH, J
SHEN, PY
机构：
[1] Department of Chemistry, Western Michigan University Kalamazoo
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 37卷 / 06期
关键词：
D O I：
10.1016/0006-291X(69)90211-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Puromycin led either to stimulation or to inhibition of phenylalanine incorporation in cell-free systems from Bacillus licheniformis and Bacillus stearothermophilus 10. Stimulation was observed at low puromycin concentrations, at high puromycin concentrations in the presence of excess transfer RNA, and over a narrow range of temperatures. Inhibition was observed at high puromycin concentrations and at temperatures below and above the range where stimulation occurred. Freshly prepared subcellular fractions were inhibited to a greater extent than stored fractions. © 1969.
引用
收藏
页码：873 / &
相关论文
共 50 条
[41]   An amino acid depleted cell-free protein synthesis system for the incorporation of non-canonical amino acid analogs into proteins [J].
Singh-Blom, Amrita ;
Hughes, Randall A. ;
Ellington, Andrew D. .
JOURNAL OF BIOTECHNOLOGY, 2014, 178 :12-22
[42]   PROTEIN-SYNTHESIS IN HUMAN LEUKOCYTES .4. MUTUAL INHIBITION OF AMINO-ACID INCORPORATION BY AMINO-ACIDS IN CELL SUSPENSIONS AND CELL-FREE SYSTEMS [J].
WINKLER, K ;
NETH, R ;
HELLERSC.G .
HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1972, 353 (05) :787-&
[43]   INHIBITION BY TETRACYCLINES OF POLYURIDYLIC ACID DIRECTED PHENYLALANINE INCORPORATION IN ESCHERICHIA COLI CELL-FREE SYSTEMS [J].
LASKIN, AI ;
CHAN, WM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1964, 14 (02) :137-&
[44]   AMINO ACID INCORPORATION IN AN ESCHERICHIA COLI CELL-FREE SYSTEM DIRECTED BY BROMEGRASS MOSAIC VIRUS RIBONUCLEIC ACID [J].
STUBBS, JD ;
KAESBERG, P .
VIROLOGY, 1967, 33 (03) :385-&
[45]   INCORPORATION OF AMINO ACIDS INTO PROTEIN BY CELL-FREE EXTRACTS OF PENICILLIUM CHRYSOGENUM [J].
HAIDLE, CW ;
KORNFELD, JM ;
KIGHT, SG .
ARCHIV FUR MIKROBIOLOGIE, 1966, 53 (01) :41-&
[46]   Incorporation of functionalized amino acids during cell-free protein expression [J].
Easton, Chris J. ;
Fraser, Samuel A. ;
Liutkus, Mantas ;
Wang, Zhongqiang .
AMINO ACIDS, 2015, 47 (08) :1656-1656
[47]   Incorporation of nonnatural amino acids into proteins in cell-free translation systems [J].
Hohsaka, Takahiro .
SEIKAGAKU, 2007, 79 (03) :247-253
[48]   Advances and Challenges in Cell-Free Incorporation of Unnatural Amino Acids Into Proteins [J].
Gao, Wei ;
Cho, Eunhee ;
Liu, Yingying ;
Lu, Yuan .
FRONTIERS IN PHARMACOLOGY, 2019, 10
[49]   INCORPORATION OF AMINO ACIDS INTO PROTEIN IN A CELL-FREE SYSTEM FROM YEAST [J].
SO, AG ;
DAVIE, EW .
BIOCHEMISTRY, 1963, 2 (01) :132-&
[50]   INCORPORATION OF AMINO ACIDS INTO CYTOCHROME C IN A BACTERIAL CELL-FREE SYSTEM [J].
MITSUI, H .
JOURNAL OF BIOCHEMISTRY, 1961, 49 (05) :409-&
12345