STIMULATION AND INHIBITION BY PUROMYCIN OF CELL-FREE AMINO ACID INCORPORATION

被引:7
作者
STENESH, J
SHEN, PY
机构
[1] Department of Chemistry, Western Michigan University Kalamazoo
关键词
D O I
10.1016/0006-291X(69)90211-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Puromycin led either to stimulation or to inhibition of phenylalanine incorporation in cell-free systems from Bacillus licheniformis and Bacillus stearothermophilus 10. Stimulation was observed at low puromycin concentrations, at high puromycin concentrations in the presence of excess transfer RNA, and over a narrow range of temperatures. Inhibition was observed at high puromycin concentrations and at temperatures below and above the range where stimulation occurred. Freshly prepared subcellular fractions were inhibited to a greater extent than stored fractions. © 1969.
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收藏
页码:873 / &
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