HISTOCHEMICAL DEMONSTRATION OF ALPHA-GLUCAN PHOSPHORYLASE ACTIVITY IN HUMAN AXILLARY APOCRINE SWEAT GLAND

The effect of glycogen and glycogen metabolites on the histochemical reaction of α-glucan phosphorylase was examined. The addition of glycogen, dextrin, amylopectin or dextran with a higher molecular weight to the substrate solution was scarcely effective in shortening incubation time necessary for the appearance of a positive reaction. In contrast, when dextran with a lower molecular weight was introduced into the substrate solution, the incubation time necessary for the appearance of the reaction was shortened. By the use of dextran-containing substrate solution, the activity of α-glucan phosphorylase in the human axillary apocrine sweat gland could be demonstrated. © 1969, Tohoku University Medical Press. All rights reserved.