EXTRACTION OF ANTIGEN-A FROM THE SKIN OF CHICKENS INFECTED WITH THE PATHOGENIC VIRUS OF MAREKS-DISEASE

被引:0
|
作者
GAGIC, M
DURISIC, S
机构
来源
ACTA VETERINARIA-BEOGRAD | 1995年 / 45卷 / 01期
关键词
ANTIGEN A; VIRUS; IMMUNITY; MAREKS DISEASE;
D O I
暂无
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The paper presents possibilities for extracting antigen ''A'' from the skin; and feather follicle epithelium of chickens with Marek's disease virus MDV. The best method for releasing large quantities of MDV was sonicated destruction of the skin cells or extraction in sarcosyl buffer. All sera from infected chickens were positive in the agar gel precipitation (AGP) test with antigen ''A'' prepared in the laboratory and with reference antigen 0157/03(Weybridge). Further work is necessary in order to purify antigen ''A'' for routine laboratory work and investigate cellular immunity.
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页码:25 / 30
页数:6
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