IN-VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF POLYALTHIA LONGIFOLIA (SONN.) THWAITE SEEDS

被引:3
作者
Thonangi, Chandi Vishala [1 ]
Akula, Annapurna [1 ]
机构
[1] Andhra Univ, AU Coll Pharmaceut Sci, Pharmacol Div, Visakhapatnam 530002, Andhra Prades, India
来源
INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH | 2018年 / 9卷 / 09期
关键词
Antioxidant; Polyalthia longifolia; Egg albumin; Diclofenac; Anti-inflammatory;
D O I
10.13040/IJPSR.0975-8232.9(9).3774-80
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The present study is designed to evaluate the in vitro antioxidant and in-vivo anti-inflammatory activities of Polyalthia longifolia (Sonn.) Thwaite seeds. Initially, the extracts (Nonpolar to polar) were screened for in-vitro antioxidant activity by DPPH, nitric oxide radical, hydroxyl radical, and superoxide radical considering ascorbic acid as standard. Among the extracts the ethyl acetate and methanolic extracts showed greater antioxidant activity further, the antioxidant activity of the extracts is supported by evaluating the total phenol content (114 +/- 1.7, 146.5 +/- 2.4 mg/g gallic acid equivalents respectively) and flavonoid content (2.86 +/- 0.10, 6.2 +/- 0.12 mg/g rutin equivalents respectively). Based on the initial study, the ethyl acetate and methanol extracts were evaluated for acute anti-inflammatory activity against the egg albumin (1%) induced inflammation. The selected extracts (200, 400 mg/kg) were administered 1 hour prior to the induction of inflammation. The anti-inflammatory activity is measured as a decrease in paw edema, measured at regular intervals (0.5, 1, 2, 3, 4, 5 h) of time, taking diclofenac (5 mg/kg) as a standard drug. Both the extracts showed significant dose-dependent anti-inflammatory activity when compared with the control. The 400 mg/kg dose of methanol extract showed greater percentage of inhibition (58.46%) when compared with the diclofenac (56.92%). The anti-inflammatory activity of the extracts is attributed due to the presence of active constituents like phenols, flavonoids, terpenes, steroids, etc. Hence Polyalthia longifolia seeds can be used in developing the lead molecules for the treatment of various chronic diseases like cancer, which are associated with the generation of ROS and inflammation.
引用
收藏
页码:3774 / 3780
页数:7
相关论文
共 32 条
  • [1] Adams CD, 2007, J ETHNOPHARMACOL, V109, P219
  • [2] Adebiyi Olamide E., 2017, BENI SUEF U J BASIC, V6, P10, DOI [10.1016/j.bjbas.2016.12.003, DOI 10.1016/j.bjbas.2016.12.003, 10.1016/j.bjbas.2016.12.003., DOI 10.1016/J.BJBAS.2016.12.003]
  • [3] Antiinflammatory activity of the aqueous leaf extract of Byrsocarpus coccineus
    Akindele, A. J.
    Adeyemi, O. O.
    [J]. FITOTERAPIA, 2007, 78 (01) : 25 - 28
  • [4] Review on in vivo and in vitro methods evaluation of antioxidant activity
    Alam, Md. Nur
    Bristi, Nusrat Jahan
    Rafiquzzaman, Md.
    [J]. SAUDI PHARMACEUTICAL JOURNAL, 2013, 21 (02) : 143 - 152
  • [5] Annan K, 2013, J MED BIOMED SCI, V2, P8
  • [6] The anti-inflammatory activity of garden egg (Solanum aethiopicum) on egg albumin-induced oedema and granuloma tissue formation in rats
    Anosike, Chioma A.
    Obidoa, Onyechi
    Ezeanyika, Lawrence U. S.
    [J]. ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE, 2012, 5 (01) : 62 - 66
  • [7] Solicitation of HPLC and HPTLC Techniques for Determination of Rutin from Polyalthia longifolia Thwaites
    Doshi, Gaurav Mahesh
    Zine, Sandeep Prabhakar
    Chaskar, Pratip Kashinath
    Une, Hemant Devidas
    [J]. PHARMACOGNOSY RESEARCH, 2014, 6 (03): : 234 - 239
  • [8] Habu JB, 2015, BIOL RES, V48, DOI [10.1186/s40659-015-0007-x, 10.1186/S40659-015-0007-X]
  • [9] Antioxidant Activity and Hepatoprotective Potential of Polyalthia longifolia and Cassia spectabilis Leaves against Paracetamol-Induced Liver Injury
    Jothy, Subramanion L.
    Aziz, Azlan
    Chen, Yeng
    Sasidharan, Sreenivasan
    [J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2012, 2012
  • [10] In vitro evaluation of antioxidant activity of some plant methanol extracts
    Karagoz, Ali
    Artun, Fulya T.
    Ozcan, Guel
    Melikoglu, Gulay
    Anil, Sezin
    Kultur, Sukran
    Sutlupinar, Nurhayat
    [J]. BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 2015, 29 (06) : 1184 - 1189