SEQUENCE OF THE 4TH AND 5TH PHOTOSYSTEM-II TYPE-I CHLOROPHYLL A/B-BINDING PROTEIN GENES OF ARABIDOPSIS-THALIANA AND EVIDENCE FOR THE PRESENCE OF A FULL COMPLEMENT OF THE EXTENDED CAB GENE FAMILY

被引:36
|
作者
MCGRATH, JM
TERZAGHI, WB
SRIDHAR, P
CASHMORE, AR
PICHERSKY, E
机构
[1] UNIV MICHIGAN,DEPT BIOL,ANN ARBOR,MI 48109
[2] UNIV PENN,DEPT BIOL,LEIDY LAB BIOL,PHILADELPHIA,PA 19104
关键词
GENE DUPLICATION; PHOTOSYNTHESIS; RFLP; SOUTHERN BLOTS;
D O I
10.1007/BF00027069
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A second locus (Lhb1B) encoding Photosystem II Type I chlorophyll a/b-binding (CAB) polypeptides was identified in Arabidopsis thaliana. This locus carries two genes in an inverted orientation. The predicted sequences of the polypeptides encoded by these two genes show substantial divergence in their amino termini relative to each other and to the proteins encoded by the three Lhb1 CAB genes previously characterized [10], but little divergence within the predicted primary structure of the mature protein. DNA probes derived from seven additional types of tomato CAB genes, encoding chlorophyll a/b-binding polypeptides of several antenna systems of the photosynthetic apparatus, were tested against A. thaliana. Each of these hybridized in Southern blots to unique DNA fragment(s), demonstrating the existence of each of these different types of CAB genes in the genome of A. thaliana. The number of genes encoding each CAB type in A. thaliana was estimated to be similar to that of tomato.
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页码:725 / 733
页数:9
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