PURIFICATION AND PROPERTIES OF A PROKARYOTE TYPE GLUTAMINE-SYNTHETASE FROM THE BIALAPHOS PRODUCER STREPTOMYCES-HYGROSCOPICUS SF1293

被引：3

作者：

KUMADA, Y ^{[1
]}

TAKANO, E ^{[1
]}

NAGAOKA, K ^{[1
]}

机构：

[1] MEIJI SEIKA KAISHA LTD,PHARMACEUT RES CTR,YOKOHAMA,KANAGAWA 222,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1990年 / 70卷 / 01期

关键词：

D O I：

10.1016/0922-338X(90)90032-R

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A prokaryote type glutamine synthetase (GS) was purified from a bialaphos (BA)-producing organism, Streptomyces hygroscopicus SF1293 (SF1293). The GS (GS I) consisted of a 55,000 dalton subunit, and its N-terminal amino acid sequence was similar to that of S. coelicolor GS. GS I was highly sensitive to GS inhibitor phosphinothricin (PPT). An increase of GS activity was observed accompanied by BA accumulation. © 1990.

引用

页码：61 / 62

页数：2

共 16 条

[1] BIOCHEMICAL PARAMETERS OF GLUTAMINE-SYNTHETASE FROM KLEBSIELLA-AEROGENES [J].

BENDER, RA ;

JANSSEN, KA ;

RESNICK, AD ;

BLUMENBERG, M ;

FOOR, F ;

MAGASANIK, B .

JOURNAL OF BACTERIOLOGY, 1977, 129 (02) :1001-1009

[2] REGULATION OF 2 FORMS OF GLUTAMINE SYNTHETASE IN ESCHERICHIA COLI BY AMMONIA CONTENT OF GROWTH MEDIUM [J].

HOLZER, H ;

SCHUTT, H ;

MASEK, Z ;

MECKE, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1968, 60 (02) :721-&

[3]

KONDO Y, 1973, Scientific Reports of Meiji Seika Kaisha, V13, P34

[4] THE BIALAPHOS RESISTANCE GENE (BAR) PLAYS A ROLE IN BOTH SELF-DEFENSE AND BIALAPHOS BIOSYNTHESIS IN STREPTOMYCES-HYGROSCOPICUS [J].

KUMADA, Y ;

ANZAI, H ;

TAKANO, E ;

MURAKAMI, T ;

HARA, O ;

ITOH, R ;

IMAI, S ;

SATOH, A ;

NAGAOKA, K .

JOURNAL OF ANTIBIOTICS, 1988, 41 (12) :1838-1845

[5]

KUMADA Y, 1990, IN PRESS J BACTERIOL

[6] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[7] THE BIALAPHOS BIOSYNTHETIC GENES OF STREPTOMYCES-HYGROSCOPICUS - MOLECULAR-CLONING AND CHARACTERIZATION OF THE GENE-CLUSTER [J].

MURAKAMI, T ;

ANZAI, H ;

IMAI, S ;

SATOH, A ;

NAGAOKA, K ;

THOMPSON, CJ .

MOLECULAR & GENERAL GENETICS, 1986, 205 (01) :42-50

[8]

OGAWA Y, 1973, Scientific Reports of Meiji Seika Kaisha, V13, P42

[9]

PARESS PS, 1985, J GEN MICROBIOL, V131, P1903

[10] STUDIES ON THE BIOSYNTHESIS OF BIALAPHOS (SF-1293) .5. PRODUCTION OF 2-PHOSPHINOMETHYLMALIC ACID, AN ANALOG OF CITRIC-ACID BY STREPTOMYCES-HYGROSCOPICUS SF-1293 AND ITS INVOLVEMENT IN THE BIOSYNTHESIS OF BIALAPHOS [J].

SETO, H ;

IMAI, S ;

SASAKI, T ;

SHIMOTOHNO, K ;

TSURUOKA, T ;

OGAWA, H ;

SATOH, A ;

INOUYE, S ;

NIIDA, T ;

OTAKE, N .

JOURNAL OF ANTIBIOTICS, 1984, 37 (11) :1509-1511

← 1 2 →