Application of Plackett-Burman Design for Optimization of Alkaline Protease and alpha-amylase Production by the Marine Bacterium Bacillus methylotrophicus SCJ4

被引：0
|
作者：
El-Gendi, Hamada [1 ]
Azab, Mohamed S. [2 ]
Soliman, Nadia A. [1 ]
Abdel-Fattah, Yasser R. [1 ]
机构：
[1] City Sci Res & Technol Applicat, Genet Engn & Biotechnol Res Inst, Bioproc Dev Dept, Alexandria, Egypt
[2] Al Azhar Univ, Fac Sci, Dept Bot & Microbiol, Cairo, Egypt
来源：
RESEARCH JOURNAL OF PHARMACEUTICAL BIOLOGICAL AND CHEMICAL SCIENCES | 2016年 / 7卷 / 04期
关键词：
Bacillus methylotrophicus; fractional factorial design; enzymes;
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Bacillus methylotrophicus SCJ4, a marine bacteria isolated from highly polluted area of El-Max bay, Egypt showed a high potency for contemporaneous production of alkaline protease and alpha-amylase enzymes. In the current study, Plackett-Burman factorial design was applied to evaluate culture conditions affecting the production of both enzymes. Analysis of Plackett-Burman design results revealed that, the most significant variables affecting alkaline proteasedium production were starch, K2HPO4, MgSO4 and CaCl2 with more than 6 folds increase in enzyme activity than basal medium (1185 Uml(-1)min(-1) after 48h), while alpha-amylase production, were glucose, starch, peptone and MgSO4 with 9 folds increase in enzyme activity than basal medium (280 Uml(-1) min(-1) 48h).
引用
收藏
页码：899 / 909
页数：11
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