COMPARISON OF EXPRESSION OF VIRULENCE FACTORS BY ESCHERICHIA-COLI CAUSING CYSTITIS AND ESCHERICHIA-COLI COLONIZING THE PERIURETHRA OF HEALTHY GIRLS

The prevalence of virulence factors possessed by Escherichia coli colonizing the periurethra was examined and compared with that of E. coli causing urinary tract infection, One hundred two E. coli isolates obtained from the periurethra and urine of 7 non-sexually active girls at risk for cystitis were characterized for genetic relatedness and examined for expression of six virulence factors. Expression of virulence factors in colonizing clones of E. coli were compared with expression in clones from symptomatic infection. All virulence factors except P adhesin were commonly expressed by colonizing clones of E. coli that did not go on to infect the urinary tract, Although P adhesin-expressing E. coli were commonly isolated from episodes of cystitis, the presence of P adhesin on periurethral E. coli did not usually predict subsequent infection. By itself, expression of virulence factors on periurethral E. coli is not enough to predict subsequent infection.