NEW AGROBACTERIUM HELPER PLASMIDS FOR GENE-TRANSFER TO PLANTS

被引：1179
作者：
HOOD, EE
GELVIN, SB
MELCHERS, LS
HOEKEMA, A
机构：
[1] MOGEN INT,2333 CB LEIDEN,NETHERLANDS
[2] PURDUE UNIV,DEPT BIOL SCI,W LAFAYETTE,IN 47907
来源：
TRANSGENIC RESEARCH | 1993年 / 2卷 / 04期
关键词：
AGROBACTERIUM; PLANT TRANSFORMATION; HELPER PLASMIDS; HOST RANGE; TI-PLASMID; T-DNA;
D O I：
10.1007/BF01977351
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We describe the construction of new helper Ti plasmids for Agrobacterium-mediated plant transformation. These plasmids are derived from three different Agrobacterium tumefaciens Ti plasmids, the octopine plasmid pTiB6, the nopaline plasmid pTiC58, and the L,L-succinamopine plasmid pTiBo542. The T-DNA regions of these plasmids were deleted using site-directed mutagenesis to yield replicons carrying the vir genes that will complement binary vectors in trans. Data are included that demonstrate strain utility. The advantages of Agrobacterium strains harbouring these 'disarmed' Ti plasmids for plant transformation via Agrobacterium are discussed.
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页码：208 / 218
页数：11
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