We describe a novel technique for counter-selection of donor cells in conjugation experiments. The technique is based on application of antibody coated magnetic beads for removal of donor cells prior to plating. This makes the technique useful in conjugation experiments where no selective growth regimes can be applied. The technique was employed in conjugation experiments where a resistance plasmid, naturally occurring in the, fish pathogenic bacterium Aeromonas salmonicida, was transferred to Escherichia coli HB101 and a marine vibrio. Use of the immunomagnetic beads Co remove donor cells prior to plating resulted in transconjugants constituting 8.9% to 90.9% (Aeromonas salmonicida) of total plate count.
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Nanchang Univ, State Key Lab Food Sci & Resources, Nanchang 330047, Peoples R China
Beijing Ctr Dis Control & Prevent, Beijing Key Lab Diagnost & Traceabil Technol Food, 16 Hepingli Middle St, Beijing 100013, Peoples R ChinaNanchang Univ, State Key Lab Food Sci & Resources, Nanchang 330047, Peoples R China
Chen, Wenyao
Huang, Zheng
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Shenzhen Third Peoples Hosp, Natl Clin Res Ctr Infect Dis, Shenzhen 518112, Guangdong, Peoples R ChinaNanchang Univ, State Key Lab Food Sci & Resources, Nanchang 330047, Peoples R China
Huang, Zheng
Cao, Wenkai
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Nanchang Univ, State Key Lab Food Sci & Resources, Nanchang 330047, Peoples R ChinaNanchang Univ, State Key Lab Food Sci & Resources, Nanchang 330047, Peoples R China
Cao, Wenkai
Peng, Juan
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Nanchang Univ, State Key Lab Food Sci & Resources, Nanchang 330047, Peoples R ChinaNanchang Univ, State Key Lab Food Sci & Resources, Nanchang 330047, Peoples R China
Peng, Juan
Lai, Weihua
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Nanchang Univ, State Key Lab Food Sci & Resources, Nanchang 330047, Peoples R ChinaNanchang Univ, State Key Lab Food Sci & Resources, Nanchang 330047, Peoples R China
Lai, Weihua
Shao, Bin
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Beijing Ctr Dis Control & Prevent, Beijing Key Lab Diagnost & Traceabil Technol Food, 16 Hepingli Middle St, Beijing 100013, Peoples R ChinaNanchang Univ, State Key Lab Food Sci & Resources, Nanchang 330047, Peoples R China
机构:
US FDA, Natl Ctr Toxicol Res, Div Microbiol, Jefferson, AR 72079 USA
Univ Arkansas, Cellular & Mol Biol Grad Program, Fayetteville, AR USAUS FDA, Natl Ctr Toxicol Res, Div Microbiol, Jefferson, AR 72079 USA
Algarni, Suad
Gudeta, Dereje D.
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US FDA, Natl Ctr Toxicol Res, Div Microbiol, Jefferson, AR 72079 USAUS FDA, Natl Ctr Toxicol Res, Div Microbiol, Jefferson, AR 72079 USA
Gudeta, Dereje D.
Sopovski, Danielle
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US FDA, Natl Ctr Toxicol Res, Div Microbiol, Jefferson, AR 72079 USAUS FDA, Natl Ctr Toxicol Res, Div Microbiol, Jefferson, AR 72079 USA
Sopovski, Danielle
Han, Jing
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US FDA, Natl Ctr Toxicol Res, Div Microbiol, Jefferson, AR 72079 USAUS FDA, Natl Ctr Toxicol Res, Div Microbiol, Jefferson, AR 72079 USA
Han, Jing
Feye, Kristina M.
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US FDA, Natl Ctr Toxicol Res, Div Microbiol, Jefferson, AR 72079 USAUS FDA, Natl Ctr Toxicol Res, Div Microbiol, Jefferson, AR 72079 USA
Feye, Kristina M.
Foley, Steven L.
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US FDA, Natl Ctr Toxicol Res, Div Microbiol, Jefferson, AR 72079 USAUS FDA, Natl Ctr Toxicol Res, Div Microbiol, Jefferson, AR 72079 USA