PHARMACOLOGICAL REVERSAL OF ACUTE CHANGES IN DIFFUSION-WEIGHTED MAGNETIC-RESONANCE-IMAGING IN FOCAL CEREBRAL-ISCHEMIA

被引:74
作者
LO, EH
MATSUMOTO, K
PIERCE, AR
GARRIDO, L
LUTTINGER, D
机构
[1] MASSACHUSETTS GEN HOSP,CTR IMAGING & PHARMACEUT RES,BOSTON,MA 02114
[2] HARVARD UNIV,SCH MED,BOSTON,MA
[3] MASSACHUSETTS GEN HOSP,MGH NMR CTR,BOSTON,MA 02114
[4] STERLING WINTHROP INC,DIV PHARMACEUT RES,NEUROSCI GRP,COLLEGEVILLE,PA
[5] NEW YORK STATE DEPT HLTH,BUR TOX SUBST ASSESSMENT,NEW YORK,NY
关键词
STROKE; DIFFUSION-WEIGHTED MRI; NEUROPROTECTION; PHARMACOTHERAPY; GLUTAMATE; RAT;
D O I
10.1038/jcbfm.1994.74
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Recently, diffusion-weighted magnetic resonance imaging (DWI) has been shown to visualize acute ischemic lesions in the brain before changes are observable with conventional magnetic resonance imaging. However, the underlying mechanisms of these acute DWI changes are unclear and may include both reversible and irreversible damage. In this study, we demonstrate that acute DWI lesions may be reversed with MK801 therapy postischemia. Sprague-Dawley rats (n = 12) were subjected to middle cerebral artery occlusion and DWI scans were obtained beginning 600 min postocclusion. Distinct regions of hyperintensity were observed in the basal ganglia and cortex, corresponding with the expected distribution of ischemia in this model. After the first scan, animals were treated with MK801 (0.5 mg/kg i.v.) or normal saline and subsequently scanned again 30 and 60 min after treatment. In the control group, the area of hyperintense lesions continued to increase, by 55% in the cortex and 57% in the basal ganglia. MK801 therapy significantly (p < 0.01) reduced the area of damage by the third DWI scan at 60 min posttreatment (-50% cortex, -22% basal ganglia, -41% total hemisphere) compared to pretreatment scans. Tetrazolium (TTC) stains at 24 h confirmed that MK801 significantly reduced the volumes of infarction (p < 0.05). These results demonstrate that significant portions of the acute ischemic lesion on DWI are reversible with pharmacologic intervention. .
引用
收藏
页码:597 / 603
页数:7
相关论文
共 28 条
  • [1] MECHANISM OF DETECTION OF ACUTE CEREBRAL-ISCHEMIA IN RATS BY DIFFUSION-WEIGHTED MAGNETIC-RESONANCE MICROSCOPY
    BENVENISTE, H
    HEDLUND, LW
    JOHNSON, GA
    [J]. STROKE, 1992, 23 (05) : 746 - 754
  • [2] SMALL DIFFERENCES IN INTRAISCHEMIC BRAIN TEMPERATURE CRITICALLY DETERMINE THE EXTENT OF ISCHEMIC NEURONAL INJURY
    BUSTO, R
    DIETRICH, WD
    GLOBUS, MYT
    VALDES, I
    SCHEINBERG, P
    GINSBERG, MD
    [J]. JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1987, 7 (06) : 729 - 738
  • [3] CAMPAGNE MV, 1993, SOC MAGN RESON IMAGI, V12, P294
  • [4] GOLDBERG MP, 1993, J NEUROSCI, V13, P3510
  • [5] HARTLEY DM, 1989, J PHARMACOL EXP THER, V250, P752
  • [6] TEMPORAL EVOLUTION OF ISCHEMIC DAMAGE IN RAT-BRAIN MEASURED BY PROTON NUCLEAR-MAGNETIC-RESONANCE IMAGING
    KNIGHT, RA
    ORDIDGE, RJ
    HELPERN, JA
    CHOPP, M
    RODOLOSI, LC
    PECK, D
    [J]. STROKE, 1991, 22 (06) : 802 - 808
  • [7] MR IMAGING OF INTRAVOXEL INCOHERENT MOTIONS - APPLICATION TO DIFFUSION AND PERFUSION IN NEUROLOGIC DISORDERS
    LEBIHAN, D
    BRETON, E
    LALLEMAND, D
    GRENIER, P
    CABANIS, E
    LAVALJEANTET, M
    [J]. RADIOLOGY, 1986, 161 (02) : 401 - 407
  • [8] IMAGING OF DIFFUSION AND MICROCIRCULATION WITH GRADIENT SENSITIZATION - DESIGN, STRATEGY, AND SIGNIFICANCE
    LEBIHAN, D
    TURNER, R
    MOONEN, CTW
    PEKAR, J
    [J]. JMRI-JOURNAL OF MAGNETIC RESONANCE IMAGING, 1991, 1 (01): : 7 - 28
  • [9] EFFECTS OF HYPOTHERMIA ON EVOKED-POTENTIALS, MAGNETIC-RESONANCE-IMAGING, AND BLOOD-FLOW IN FOCAL ISCHEMIA IN RABBITS
    LO, EH
    STEINBERG, GK
    [J]. STROKE, 1992, 23 (06) : 889 - 893
  • [10] EFFECTS OF NMDA AND CALCIUM-CHANNEL ANTAGONISTS ON REGIONAL CEREBRAL BLOOD-FLOW
    LO, EH
    SUN, GH
    STEINBERG, GK
    [J]. NEUROSCIENCE LETTERS, 1991, 131 (01) : 17 - 20