The combination of the expression of hexokinase 2 and pyruvate kinase M2 is a prognostic marker in patients with pancreatic cancer

被引：60
作者：
Ogawa, Hisataka [1 ,2 ]
Nagano, Hiroaki [1 ]
Konno, Masamitsu [2 ]
Eguchi, Hidetoshi [1 ]
Koseki, Jun [3 ]
Kawamoto, Koichi [1 ,2 ]
Nishida, Naohiro [1 ,2 ]
Colvin, Hugh [1 ]
Tomokuni, Akira [1 ]
Tomimaru, Yoshito [1 ]
Hama, Naoki [1 ]
Wada, Hiroshi [1 ]
Marubashi, Shigeru [1 ]
Kobayashi, Shogo [1 ]
Mori, Masaki [1 ]
Doki, Yuichiro [1 ]
Ishii, Hideshi [2 ,3 ]
机构：
[1] Osaka Univ, Grad Sch Med, Dept Gastroenterol Surg, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan
[2] Osaka Univ, Grad Sch Med, Frontier Sci Canc & Chemotherapy, Suita, Osaka 5650871, Japan
[3] Osaka Univ, Grad Sch Med, Canc Profiling Discovery, Suita, Osaka 5650871, Japan
来源：
MOLECULAR AND CLINICAL ONCOLOGY | 2015年 / 3卷 / 03期
关键词：
hexokinase; pyruvate kinase; cancer metabolism; pancreatic cancer;
D O I：
10.3892/mco.2015.490
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Metabolism may determine the biologically malignant behavior of pancreatic cancer. To investigate the significance and prognostic value of cancer metabolism in cancer patients, we investigated the expression of two key enzymes in anaerobic glycolysis, hexokinase 2 (HK2) and pyruvate kinase isoenzyme type M2 (PKM2), in surgical specimens obtained from 36 patients who underwent curative resection of pancreatic ductal carcinoma. The HK2-glycolysis axis is a key system in the clinical imaging of tumors via positron emission tomography. Immunohistochemical staining for HK2 and PKM2 was performed and the data were statistically analyzed to evaluate their prognostic power. The expression of HK2 and PKM2 was associated with clinicopathological variables and patient prognosis, including overall survival, local recurrence-free survival and distant metastasis-free survival. Staining for HK2 was negative and positive in 42 and 58% of the patients, respectively, whereas staining for PKM2 was negative and positive in 56 and 44%, respectively; HK2-positive staining was correlated with progressive pathological tumor stage (pT3 vs. pT1 and pT2; P=0.017). In the univariate analysis, the positive expression of HK2 and PKM2, pathological stage (pT3 vs. pT1 and pT2) and nodal metastasis were significantly correlated with poor prognosis (P<0.03). In the multivariate analysis, pathological nodal metastasis was an independent prognostic factor for overall survival, whereas the positive expression of HK2 and PKM2 exhibited borderline significance (P=0.08 and 0.12, hazard ratio =2.57 and 2.16, respectively). In addition, the combination of high expression of HK2 as well as PKM2 was found to be significant (P<0.05). These results suggested that the expression of HK2 and PKM2, particularly their combination, in surgical specimens obtained during curative resection, may predict an unfavorable clinical outcome in patients with pancreatic cancer.
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收藏
页码：563 / 571
页数：9
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