HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROCHEMICAL DETECTION OF 3-METHYLHISTIDINE IN HUMAN URINE

被引：8
|
作者：
BETTO, P [1 ]
RICCIARELLO, G [1 ]
PICHINI, S [1 ]
STROLOGO, LD [1 ]
RIZZONI, G [1 ]
机构：
[1] BAMBINO GESU PEDIAT HOSP,SCI RES INST,DIV NEPHROL & DIALYSIS,I-00165 ROME,ITALY
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 584卷 / 02期
关键词：
D O I：
10.1016/0378-4347(92)80583-C
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A reversed-phase high-performance liquid chromatographic method is described for the determination of 3-methylhistidine content in human urine using pre-column derivatization with phenylisothiocyanate, isocratic elution with 15 mM sodium acetate-acetonitrile (92:8, v/v) and electrochemical detection. The limit of quantitation was 0.1 pmol. The method has been applied in routine analyses of 3-methylhistidine in both clinical and research work.
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页码：256 / 260
页数：5
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