HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROCHEMICAL DETECTION OF 3-METHYLHISTIDINE IN HUMAN URINE

被引：8

作者：

BETTO, P ^{[1
]}

RICCIARELLO, G ^{[1
]}

PICHINI, S ^{[1
]}

STROLOGO, LD ^{[1
]}

RIZZONI, G ^{[1
]}

机构：

[1] BAMBINO GESU PEDIAT HOSP,SCI RES INST,DIV NEPHROL & DIALYSIS,I-00165 ROME,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 584卷 / 02期

关键词：

D O I：

10.1016/0378-4347(92)80583-C

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A reversed-phase high-performance liquid chromatographic method is described for the determination of 3-methylhistidine content in human urine using pre-column derivatization with phenylisothiocyanate, isocratic elution with 15 mM sodium acetate-acetonitrile (92:8, v/v) and electrochemical detection. The limit of quantitation was 0.1 pmol. The method has been applied in routine analyses of 3-methylhistidine in both clinical and research work.

引用

页码：256 / 260

页数：5

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