PRESENCE OF IMMUNOREACTIVE ALPHA-ENDORPHIN IN RAT PITUITARY-GLAND

被引：5
作者：
FUKATA, J
NAKAI, Y
IMURA, H
机构：
[1] 2nd Division, Department of Internal Medicine, Kyoto University Faculty of Medicine, Kyoto
来源：
LIFE SCIENCES | 1979年 / 25卷 / 06期
关键词：
D O I：
10.1016/0024-3205(79)90568-X
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Utilizing radioimmunoassay for α-endorphin, we attempted to demonstrate immunoreactive α-endorphin in acid extracts of pars distalis and combined pars intermedia and pars nervosa of the rat pituitary gland after chromatography on Sephadex G-25. β-Lipotropin, β-endorphin and γ-endorphin were not converted into α-endorphin during the extraction and gel chromatographic procedures. Concentrations of immunoreactive α-endorphin determined after gel chromatography of extracts from pars distalis and combined pars intermedia and pars nervosa were 1.1±0.6 and 130±17 ng/mg wet tissue (mean±SE), respectively. Serial dilution of these extracts gave parallel lines to the standard curve of synthetic α-endorphin, but not to that of γ-endorphin or δ-endorphin. These results suggest the existence of immunoreactive α-endorphin indistinguishable in molecular size from synthetic α-endorphin in the rat pituitary gland. © 1979.
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页码：541 / 546
页数：6
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