TRANSMITTER RELEASE AND PRESYNAPTIC CA2+ CURRENTS BLOCKED BY THE SPIDER TOXIN OMEGA-AGA-IVA

被引：111

作者：

PROTTI, DA ^{[1
]}

UCHITEL, OD ^{[1
]}

机构：

[1] UNIV BUENOS AIRES, FAC MED, INST CELL BIOL, RA-1121 BUENOS AIRES, ARGENTINA

来源：

NEUROREPORT | 1993年 / 5卷 / 03期

关键词：

CALCIUM CHANNELS; P-TYPE CHANNELS; TRANSMITTER RELEASE; OMEGA-AGA-IVA; FUNNEL WEB SPIDER TOXINS; CALCIUM CHANNEL BLOCKERS; PRESYNAPTIC CURRENTS; NEUROMUSCULAR JUNCTION; SYNAPTIC TRANSMISSION;

D O I：

10.1097/00001756-199312000-00039

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

MAMMALIAN neuromuscular transmission is resistant to L and N type calcium channel blockers but very sensitive to a low molecular weight funnel web spider venom toxin, FTX, which selectively blocks P type calcium channels. To further characterize the calcium channels involved in neuromuscular transmission we studied the effect of omega Agatoxin (omega-Aga-IVA) a polypeptide P type channel blocker from the same spider venom. We show that omega-Aga-IVA is a potent and irreversible inhibitor of the presynaptic Ca2+ currents and of acetylcholine release induced by electrical stimulation or by K+ depolarization. This provides further evidences that transmitter release at the mammalian neuromuscular junction is mediated by P type Ca2+ channels.

引用

页码：333 / 336

页数：4

共 21 条

[1] OMEGA-CONOTOXIN DOES NOT BLOCK THE VERAPAMIL-SENSITIVE CALCIUM CHANNELS AT MOUSE MOTOR-NERVE TERMINALS [J].

ANDERSON, AJ ;

HARVEY, AL .

NEUROSCIENCE LETTERS, 1987, 82 (02) :177-180

[2] THE LEVATOR AURIS LONGUS MUSCLE OF THE MOUSE - A CONVENIENT PREPARATION FOR STUDIES OF SHORT-TERM AND LONG-TERM PRESYNAPTIC EFFECTS OF DRUGS OR TOXINS [J].

ANGAUTPETIT, D ;

MOLGO, J ;

CONNOLD, AL ;

FAILLE, L .

NEUROSCIENCE LETTERS, 1987, 82 (01) :83-88

[3]

ATCHISON WD, 1989, J PHARMACOL EXP THER, V251, P672

[4] PROPERTIES OF CALCIUM CHANNELS ISOLATED WITH SPIDER TOXIN, FTX [J].

CHERKSEY, BD ;

SUGIMORI, M ;

LLINAS, RR .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES-SERIES, 1991, 635 :80-89

[5] KINETIC AND PHARMACOLOGICAL PROPERTIES DISTINGUISHING 3 TYPES OF CALCIUM CURRENTS IN CHICK SENSORY NEURONS [J].

FOX, AP ;

NOWYCKY, MC ;

TSIEN, RW .

JOURNAL OF PHYSIOLOGY-LONDON, 1987, 394 :149-172

[6] THE ANTAGONISM BETWEEN BOTULINUM TOXIN AND CALCIUM IN MOTOR-NERVE TERMINALS [J].

GUNDERSEN, CB ;

KATZ, B ;

MILEDI, R .

PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1982, 216 (1204) :369-376

[7]

Katz B., 1969, SHERRINGTON LECT

[8] BLOCKING AND ISOLATION OF A CALCIUM-CHANNEL FROM NEURONS IN MAMMALS AND CEPHALOPODS UTILIZING A TOXIN FRACTION (FTX) FROM FUNNEL-WEB SPIDER POISON [J].

LLINAS, R ;

SUGIMORI, M ;

LIN, JW ;

CHERKSEY, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (05) :1689-1693

[9] DEPOLARIZATION RELEASE COUPLING - AN OVERVIEW [J].

LLINAS, RR .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES-SERIES, 1991, 635 :3-17

[10] ELECTRIC-CURRENT FLOW INSIDE PERINEURIAL SHEATHS OF MOUSE MOTOR NERVES [J].

MALLART, A .

JOURNAL OF PHYSIOLOGY-LONDON, 1985, 368 (NOV) :565-+

← 1 2 3 →