MODEL STUDIES OF THE IRON-CATALYZED HABER-WEISS CYCLE AND THE ASCORBATE-DRIVEN FENTON REACTION

被引：156

作者：

BURKITT, MJ ^{[1
]}

GILBERT, BC ^{[1
]}

机构：

[1] UNIV YORK,DEPT CHEM,YORK YO1 5DD,N YORKSHIRE,ENGLAND

来源：

FREE RADICAL RESEARCH COMMUNICATIONS | 1990年 / 10卷 / 4-5期

关键词：

Ascorbate; Haber-Weiss cycle; Hydroxylation assays; Iron; Stopped-flow e.s.r. spectroscopy;

D O I：

10.3109/10715769009149895

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Complementary hydroxylation assays and stopped-flow e.s.r. techniques have been employed in the investigation of the effect of various iron chelators (of chemical, biological and clinical importance) on hydroxyl-radical generation via the Haber-Weiss cycle and the ascorbate-driven Fenton reaction. Chelators have been identified which selectively promote or inhibit various reactions involved in hydroxyl-radical generation (for example, NTA and EDTA promote all the reactions of both the Haber-Weiss cycle and the ascorbate-driven Fenton reaction, whereas DTPA and phytate inhibit the recycling of iron in these reactions). The biological chelators succinate and citrate are shown to be relatively poor catalysts of the Haber-Weiss cycle, whereas they are found to be effective catalysts of ·OH generation in the ascorbate-driven Fenton reaction. It is also suggested that continuous redox-cycling reactions between iron, oxygen and ascorbate may represent an important mechanism of cell death in biological systems. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：265 / 280

页数：16

共 46 条

[1] MECHANISM OF REDUCTION OF SINGLE-SITE REDOX PROTEINS BY ASCORBIC-ACID [J].