DETERMINATION OF GLYCYRRHETINIC ACID IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：15

作者：

BROWNTHOMAS, JM

CHRISTENSEN, RG

RIEGER, R

MALONE, W

MAY, WE

机构：

[1] Organic Analytical Research Division, Center for Analytical Chemistry, National Institute of Standards and Technology, Gaithersburg

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 568卷 / 01期

关键词：

D O I：

10.1016/0378-4347(91)80358-J

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic (HPLC) method has been developed for measuring 18-beta-glycyrrhetinic acid (GRA) in human plasma in the range of 0.1-3-mu-g/ml. The acetate ester of GRA is added to the plasma as an internal standard, plasma proteins are denatured with urea to release GRA, and the GRA and the internal standard are extracted in an ion-pairing solid-phase extraction process. An isocratic, reversed-phase HPLC separation is used, followed by ultraviolet absorbance detection at 248 nm. The results from the analysis of five GRA-fortified plasma pools show a mean relative standard deviation of 7% and are accurate to within 10%. With evaporative concentration of the extract, the limit of detection for GRA in plasma is approximately 10 ng/ml.

引用

页码：232 / 238

页数：7

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