THE SYNTHESIS OF ALPHA-([C-11]METHYL)PHENYLALANINE AND ALPHA-([C-11]METHYL)TYROSINE FROM C-11 LABELED MALONIC ESTERS

The synthesis of alpha-([C-11]methyl)tyrosine 4a and alpha-([C-11]methyl)phenylalanine 4b from dimethyl 2-(4-methoxybenzyl)-2-([C-11]methyl)malonate 1a and dimethyl 2-benzyl-2-([C-11]methyl)malonate 1b, respectively is reported. After the alkylation of dimethyl 2-(4-methoxybenzyl)malonate or dimethyl 2-benzylmalonate with [C-11]methyl iodide, a selective enzymatic hydrolysis to monomethyl 2-(4-methoxybenzyl)-2-([C-11]methyl)malonic acid 2a or monomethyl 2-benzyl-2-([C-11]methyl)molanic acid 2b was carried out using pig liver esterase (EC 3.1.1.1). Conversion of the carboxylic acid moiety of 2a, b into the corresponding isocyanate 3a, b was achieved by a modified Curtius rearrangement using diphenylphosphoryl azide (DPPA). The simultaneous hydrolysis of the isocyanate and ester moieties of 3a, b using conc. hydrochloric acid produced the desired amino acids 4a, b. In the case of alpha-([C-11]methyl)tyrosine, the methyl ether protecting group was removed using trimethylsilyl chloride-sodium iodide prior to acidic hydrolysis. The amino acids were obtained in 12-20% decay corrected radiochemical yields in a synthesis time of 45-50 min, counted from the start of [C-11]methyl iodide synthesis, and with a radiochemical purity greater than 98% after preparative HPLC purification. Enantiomeric purities of 4a, b were determined by the HPLC separation of the respective 1-fluoro-5-(2,4-dinitrophenyl)-L-alanine amide (FDAA) diastereo-isomers, which showed an enantiomeric excess of 62% for 4a but no observable enantiomeric induction for 4b. To confirm the position of labelling, a C-13 synthesis was carried out using (C-13)methyl iodide in the same reaction sequence, and the product analysed by C-13 NMR spectroscopy.