EFFECTS OF PLATINUM COMPLEXES ON 7 ENZYMES

被引：59

作者：

AULL, JL ^{[1
]}

ALLEN, RL ^{[1
]}

BAPAT, AR ^{[1
]}

DARON, HH ^{[1
]}

FRIEDMAN, ME ^{[1
]}

WILSON, JF ^{[1
]}

机构：

[1] AUBURN UNIV,DEPT ANIM & DAIRY SCI,AUBURN,AL 36830

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 571卷 / 02期

关键词：

Enzyme activity; Platinum complex;

D O I：

10.1016/0005-2744(79)90105-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of K2PtCl4, cis-Pt(NH3)2Cl2, and trans-Pt(NH3)2Cl2 on the activities of glyceraldehyde-3-phosphate dehydrogenase, glucose-6-phosphate dehydrogenase, dihydrofolate reductase, fructose-1,6-bisphosphate aldolase, catalase, tyrosinase, and peroxidase have been investigated. All of the enzymes which are thought to have essential sulfhydryl groups (glyceraldehyde-3-phosphate dehydrogenase, aldolase, and glucose-6-phosphate dehydrogenase) were significantly inhibited by K2PtCl4. The other four enzymes studied are not known to have essential sulfhydryl groups, and were not significantly affected by the Pt compounds under the conditions employed. Glyceraldehyde-3-phosphate dehydrogenase was the only enzyme inhibited by all three Pt compounds tested, with K2PtCl4 being the most effective and cis-Pt(NH3)2Cl2 the least effective inhibitor. Semilogarithmic plots of residual activity versus inhibition time indicated that the inhibition reactions were not simple first-order processes, except for the inhibition of glucose-6-phosphate dehydrogenase by K2PtCl4 which appeared to be first-order with respect to enzyme concentration. © 1979.

引用

页码：352 / 358

页数：7

共 21 条

[1] INTERACTIONS OF PLATINUM COMPLEXES WITH ESSENTIAL AND NONESSENTIAL SULFHYDRYL-GROUPS OF THYMIDYLATE SYNTHETASE [J].