INTERACTION OF TRYPANOSOMA-CRUZI WITH CELLS WITH ALTERED GLYCOSYLATION PATTERNS

被引：36

作者：

CIAVAGLIA, MD ^{[1
]}

DECARVALHO, TU ^{[1
]}

DESOUZA, W ^{[1
]}

机构：

[1] UNIV FED RIO DE JANEIRO,INST BIOFIS CARLOS CHAGAS FILHO,LAB ULTRAESTRUTURA CELULAR HERTHA MEYER,BR-21949 RIO JANEIRO,BRAZIL

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 193卷 / 02期

关键词：

D O I：

10.1006/bbrc.1993.1684

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glycosylation mutants of chinese hamster ovary cells were used to analyze the role played by surface-exposed carbohydrates on the process of interaction of Trypanosoma cruzi with the host cell. Adhesion and invasion of the parasites were markedly reduced in cells which express very few sialic acid residues. Infection levels similar to those obtained with the parental cell could be obtained after sialylation of the mutant cell using exogenous fetuin as sialic acid donor and T. cruzi trans-sialidase. The results obtained show that host cell sialic acid residues are involved in the process of attachment to and penetration of T. cruzi into the host cell. © 1993 Academic Press, Inc.

引用

页码：718 / 721

页数：4

共 21 条

[1]

ARAUJO-JORGE T C D, 1989, Memorias do Instituto Oswaldo Cruz, V84, P441

[2]

ARAUJOJORGE TC, 1984, ACTA TROP BASEL, V41, P17

[3]

BRENER Z, 1973, ANNU REV MICROBIOL, V27, P17

[4]

CAVALLESCO R, 1988, J IMMUNOL, V140, P617

[5] CELL BIOLOGY OF TRYPANOSOMA-CRUZI [J].

DESOUZA, W .

INTERNATIONAL REVIEW OF CYTOLOGY-A SURVEY OF CELL BIOLOGY, 1984, 86 :197-283

[6]

DETITTO EH, 1987, ACTA TROP, V44, P273

[7] EFFECT OF POLY-L-LYSINE AND NEURAMINIDASE ON THE INFECTIVITY OF TRYPANOSOMA-CRUZI IN CULTURED HELA-CELLS [J].

GAMARRO, F ;

CASTANYS, S ;

RUIZPEREZ, LM ;

ADROHER, FJ ;

OSUNA, A .

ZEITSCHRIFT FUR PARASITENKUNDE-PARASITOLOGY RESEARCH, 1985, 71 (04) :429-433

[8]

HARTH G, 1987, P NATL ACAD SCI USA, V84, P5320

[9] ENZYMATIC TREATMENT TRANSFORMS TRYPOMASTIGOTES OF TRYPANOSOMA-CRUZI INTO ACTIVATORS OF ALTERNATIVE COMPLEMENT PATHWAY AND POTENTIATES THEIR UPTAKE BY MACROPHAGES [J].

KIPNIS, TL ;

DAVID, JR ;

ALPER, CA ;

SHER, A ;

DASILVA, WD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (01) :602-605

[10] A NEURAMINIDASE FROM TRYPANOSOMA-CRUZI REMOVES SIALIC-ACID FROM THE SURFACE OF MAMMALIAN MYOCARDIAL AND ENDOTHELIAL-CELLS [J].

LIBBY, P ;

ALROY, J ;

PEREIRA, MEA .

JOURNAL OF CLINICAL INVESTIGATION, 1986, 77 (01) :127-135

← 1 2 3 →