Purification of Plasmid DNA Using Anion-Exchange Chromatography and Removal of Endotoxin

被引：2

作者：

Pi Wenhui ^{[1
,2
]}

Sun Congjian ^{[3
]}

Song Zhiqiang ^{[2
]}

Ma Lianying ^{[3
]}

Liu Shuping ^{[3
]}

Bai Dingping ^{[2
]}

Cai Yidong ^{[3
]}

Liu Shouren ^{[2
]}

机构：

[1] Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832003, Peoples R China

[2] Xinjiang Acad Agr & Reclamat Sci, Inst Anim Husb & Vet Med, Shihezi 832003, Peoples R China

[3] Xinjiang Tianwu Sci & Technol Dev Co Ltd, Urumqi 830011, Peoples R China

来源：

CHINESE JOURNAL OF CHROMATOGRAPHY | 2007年 / 25卷 / 06期

关键词：

anion-exchange chromatography; purification; plasmid DNA; Triton X-114; Triton X-100; endotoxin; removal;

D O I：

10.1016/S1872-2059(08)60003-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The plasmid DNA was purified using the Fractogel (R) EMD TMAE M anion exchange media. The dynamic loading capacity of this media for plasmid DNA was 0. 62 mg/mL. It was found that when the lysate of bacteria was incubated with either Triton X-114 or Triton X-100 before purification the average content of endotoxin left in the purified plasmid DNA was 6. 42 EU/mg or 9.50 EU/mg respectively. These results were much lower than that without pre-incubation 67. 82 EU/mg. The purification of plasmid DNA and the removal of endotoxin were achieved in one step using anion-exchange chromatography. This method is simple fast and of low cost.

引用

页码：809 / 813

页数：5

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