SEPARATION OF IMMUNOLOGICALLY ACTIVE FRAGMENTS BY MEMBRANE PARTITION CHROMATOGRAPHY

被引：19

作者：

ZIPILIVAN, EM

HUDSON, BG

BLATT, WF

机构：

[1] Biochemistry and Pharmacology Laboratory, United States Army Research Institute of Environmental Medicine, Natick

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 30卷 / 01期

关键词：

D O I：

10.1016/0003-2697(69)90376-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A system has been described for the separation of the partially degraded products of an immunoreactive preparation using acrylamide-entrapped enzyme, and a tandem series of ultrafiltration cells, each equipped with a different molecular size retentive filter. This technique of digestion with concomitant ultrafiltration affords a means of readily obtaining immunologically reactive fragments of intermediate size. Verification of the changes in antigenicity was obtained by immunodiffusion and immunoelectrophoresis of the membrane-separated components of the enzyme-degraded mixture. © 1969.

引用

页码：91 / +

页数：1

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