SEPARATION OF ACIDIC BARLEY ENDOSPERM PROTEINS BY 2-DIMENSIONAL ELECTROPHORESIS

被引：24
作者：
FLENGSRUD, R
机构：
[1] Department of Biotechnological Sciences, Agricultural University of Norway, Ås
来源：
ELECTROPHORESIS | 1993年 / 14卷 / 10期
关键词：
D O I：
10.1002/elps.11501401169
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Proteins in barley aleurone and starchy endosperm from developing seeds were separated by two-dimensional (2-D) electrophoresis according to O'Farrell's method. The effect of different extraction methods on the separation was compared. The two best extraction methods were compared by amino acid analysis of extracts and residues. Some tissue-specific proteins in both tissues are described. Different methods for transfer of separated proteins onto membranes were compared. Amino terminal and internal sequences were determined after transfer of separated proteins or their proteolytic products onto membranes. Amino acid sequence data allowed the identification of 12 different proteins, while 6 remain unidentified and 8 appeared to have a blocked amino terminal.
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收藏
页码：1060 / 1066
页数：7
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