RAPID DIAGNOSIS OF INFLUENZA-A - COMPARISON WITH ELISA IMMUNOCAPTURE AND CULTURE

被引：21

作者：

CHOMEL, JJ ^{[1
]}

REMILLEUX, MF ^{[1
]}

MARCHAND, P ^{[1
]}

AYMARD, M ^{[1
]}

机构：

[1] BECTON DICKINSON,DIV DIAGNOST,PONT DE CLAIX,FRANCE

来源：

JOURNAL OF VIROLOGICAL METHODS | 1992年 / 37卷 / 03期

关键词：

RAPID DIAGNOSIS OF INFLUENZA-A; ELISA IMMUNOCAPTURE; CULTURES; DIRECTIGEN FLU-A;

D O I：

10.1016/0166-0934(92)90033-A

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Directigen Flu-A is an enzyme immunoassay for detecting in 15 min the influenza A nucleoproteinic antigen directly from specimens after passive adsorption on a cellulose membrane. The test was assessed using 160 frozen (-20-degrees-C) specimens collected during the 1988-1989 A/H1N1 influenza epidemic and the 1989-1990 A/H3N2 epidemic. Compared to the ELISA immunocapture test, the sensitivity of the commercial test was 87.8% and the specificity was 97.6%. When compared to isolation of viruses on LLCMK2 cells and/or chicken embryo, the sensitivity was 84%. No cross-reaction was found with other respiratory disease viruses. The feasibility, practicability and rapidity of the test make it a test of choice for rapid diagnosis of influenza A.

引用

页码：337 / 344

页数：8

共 14 条

[1]

AYMARD M, 1975, DIAGNOSTIC MALADIES, P339

[2] RAPID DIAGNOSIS OF INFLUENZA INFECTION OF NP ANTIGEN USING AN IMMUNOCAPTURE ELISA TEST [J].