SEX-SPECIFIC SPLICING AND POLYADENYLATION OF DSX PRE-MESSENGER-RNA REQUIRES A SEQUENCE THAT BINDS SPECIFICALLY TO TRA-2 PROTEIN INVITRO

被引：217
作者：
HEDLEY, ML
MANIATIS, T
机构：
[1] Harvard University, Department of Biochemistry and Molecular Biology Cambridge
来源：
CELL | 1991年 / 65卷 / 04期
关键词：
D O I：
10.1016/0092-8674(91)90090-L
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Somatic sex determination in Drosophila involves a hierarchy of regulated alternative pre-mRNA processing. Female-specific splicing and/or polyadenylation of doublesex (dsx) pre-mRNA, the final gene in this pathway, requires transformer (tra) and transformer-2 (tra-2) proteins. The mechanism by which these proteins regulate RNA processing has not been characterized. In this paper we show that tra-2 produced in Escherichia coli binds specifically to a site within the female-specific exon of dsx pre-mRNA. This site, which contains six copies of a 13 nucleotide repeat, is required not only for female-specific splicing, but also for female-specific polyadenylation. These observations suggest that tra-2 is a positive regulator of dsx pre-mRNA processing.
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收藏
页码：579 / 586
页数：8
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