Effects of Nigella sativa Seeds and its Oils Fraction on Some Biochemical Parameters in alloxan Diabetic Rats

The aim of the research study was extended to establish the chemical Composition of Nigella Saliva Seeds and to evaluate the effect of its administration and its oils fraction on serum glucose, total cholesterol, and LDL-Cholesterol of rats suffering from alloxan diabetes. Composition analysis revealed that it contains appreciable quantities of carbohydrates, protein, and fat. Minerals content showed high concentrations of phosphorus (620mg/100gm dried wt.), calcium (202 mg /100gm) and potassium (170mg /100gm). Whilst considerable amount of trace elements such, as iron, Zinc and copper. Characterization of fixed oil enumerated that unsaturated fatty acids linoleic acid and oleic acid are the dominating fraction i. e55.7mg/100gm and 24gm /100g as Compared to Saturated fatty acids palmitic acid and stearic acid i. e 13g/100g and 2.82g /100g. Hyperglycemia was induced in the rats by intraperitoneal injection of alloxan at dose of 125mg/kg body weight. Fifty of alloxan diabatic rats were divided to 5groups, each contain 10 rats of both sex. the 1st group was kept on basal diet without any treatment and considered as control (diabetic) The other four groups were taken a basal diet with 5% Nigella Sativa seeds, 2.5% crude oil, 2.5% fixed oil and volatile oil(3mg TQ\ kg body weight), respectively. Body Wt. gain of the rats did not show any statistical differences by using all the tested diet after 4and 8 weeks (the end of experiment) as compared with the diabetic control. While the food intake was significantly decrease. The levels of serum glucose, total cholesterol and LDL-cholesterol were significantly decreased on diabatic rats after treatment