USE OF A HPAII-POLYMERASE CHAIN-REACTION ASSAY TO STUDY DNA METHYLATION IN THE PGK-1 CPG ISLAND OF MOUSE EMBRYOS AT THE TIME OF X-CHROMOSOME INACTIVATION

被引：162
作者：
SINGERSAM, J
GRANT, M
LEBON, JM
OKUYAMA, K
CHAPMAN, V
MONK, M
RIGGS, AD
机构：
[1] UNIV LONDON UNIV COLL,MRC,MAMMALIAN DEV UNIT,LONDON NW1 2HE,ENGLAND
[2] NEW YORK STATE DEPT HLTH,ROSWELL PK MEM INST,DEPT MOLEC & CELLULAR BIOL,BUFFALO,NY 14263
来源：
MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 09期
关键词：
D O I：
10.1128/MCB.10.9.4987
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A HpaII-PCK assay was used to study DNA methylation in individual mouse embryos. It was found that HpaII site H-7 in the CpG island of the X-chromosome-linked Pgk-1 gene is ≤10% methylated in oocytes and male embryos but becomes 40% methylated in female embryos at 6.5 days, about the time of X-chromosome inactivation of the inner cell mass.
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页码：4987 / 4989
页数：3
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