THE INFLUENCE OF PRESSURE AND TEMPERATURE OF COMPRESSED CO2 ON THE SURVIVAL OF YEAST-CELLS

被引：133

作者：

ISENSCHMID, A ^{[1
]}

MARISON, IW ^{[1
]}

VONSTOCKAR, U ^{[1
]}

机构：

[1] SWISS FED INST TECHNOL, CH-1015 LAUSANNE, SWITZERLAND

来源：

JOURNAL OF BIOTECHNOLOGY | 1995年 / 39卷 / 03期

关键词：

SUPERCRITICAL CO2; EXTRACTION; YEAST; VIABILITY; PRESSURE SENSITIVITY;

D O I：

10.1016/0168-1656(95)00018-L

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In order to study the potential use of supercritical CO2 extraction for the recovery of products from yeast cell cultures, the effects of CO2 on different yeast strains over a range of pressures and temperatures have been examined. Viability was shown to be dependent on temperature and dissolved CO2 concentration, and can be described by a sigmoidal (S-shaped) curve. Cell death was mainly due to an 'anaesthesia effect' rather than cell rupture. Important differences in sensitivity were observed for the strains studied, with the following order of resistance: Kluyveromyces fragilis > Saccharomyces cerevisiae > Candida utilis.

引用

页码：229 / 237

页数：9

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