BIOCHEMICAL-CHARACTERIZATION OF A SPEARMINT MUTANT THAT RESEMBLES PEPPERMINT IN MONOTERPENE CONTENT

被引:59
作者
CROTEAU, R [1 ]
KARP, F [1 ]
WAGSCHAL, KC [1 ]
SATTERWHITE, DM [1 ]
HYATT, DC [1 ]
SKOTLAND, CB [1 ]
机构
[1] WASHINGTON STATE UNIV, CTR IRRIGATED AGR RES & EXTENS, PROSSER, WA 99350 USA
关键词
D O I
10.1104/pp.96.3.744
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A radiation-induced mutant of Scotch spearmint (Mentha x gracilis) was shown to produce an essential oil containing principally C3-oxygenated p-menthane monoterpenes that are typical of peppermint, instead of the C6-oxygenated monoterpene family characteristic of spearmint. In vitro measurement of all of the enzymes responsible for the production of both the C3-oxygenated and C6-oxygenated families of monoterpenes from the common precursor (-)-limonene indicated that a virtually identical complement of enzymes was present in wild type and mutant, with the exception of the microsomal, cytochrome P-450-dependent (-)-limonene hydroxylase; the C6-hydroxylase producing (-)-trans-carveol in the wild type had been replaced by a C3-hydroxylase producing (-)-trans-isopiperitenol in the mutant. Additionally, the mutant, but not the wild type, could carry out the cytochrome P-450-dependent epoxidation of the alpha,beta-unsaturated bond of the ketones formed via C3-hydroxylation. Although present in the wild type, the enzymes of the C3-pathway that convert trans-isopiperitenol to menthol isomers are synthetically inactive because of the absence of the key C3-oxygenated intermediate generated by hydroxylation of limonene. These results, which clarify the origins of the C3- and C6-oxygenation patterns, also allow correction of a number of earlier biogenetic proposals for the formation of monoterpenes in Mentha.
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页码:744 / 752
页数:9
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