THYMIC TUMORIGENESIS INDUCED BY OVEREXPRESSION OF P56LCK

被引：182
作者：
ABRAHAM, KM
LEVIN, SD
MARTH, JD
FORBUSH, KA
PERLMUTTER, RM
机构：
[1] UNIV WASHINGTON,DEPT IMMUNOL,SEATTLE,WA 98195
[2] UNIV WASHINGTON,DEPT BIOCHEM,SEATTLE,WA 98195
[3] UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98195
[4] UNIV BRITISH COLUMBIA,BIOMED RES CTR,VANCOUVER V6T 1W5,BC,CANADA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 09期
关键词：
TYROSINE KINASES; ONCOGENESIS; TRANSGENIC MICE;
D O I：
10.1073/pnas.88.9.3977
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The lck gene encodes a membrane-associated protein tyrosine kinase (p56lck) that is believed to participate in lymphocyte-specific signal transduction pathways. To investigate the function of this molecule, transgenic mice were generated carrying the wild-type lck gene or a mutated lck gene encoding a constitutively activated form of p56lck (p56lckF505). Transgene expression in thymocytes was achieved in each case using the lck proximal promoter element. Mice expressing high levels of either p56lckF505 or p56lckY505 reproducibly developed thymic tumors. The sensitivity of thymocytes to p56lck-induced transformation suggests that disturbances in lck expression may contribute to the pathogenesis of some human neoplastic diseases.
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收藏
页码：3977 / 3981
页数：5
相关论文
共 40 条
[1]   TRANSCRIPTIONAL ACTIVATION OF LCK BY RETROVIRUS PROMOTER INSERTION BETWEEN 2 LYMPHOID-SPECIFIC PROMOTERS [J].
ADLER, HT ;
REYNOLDS, PJ ;
KELLEY, CM ;
SEFTON, BM .
JOURNAL OF VIROLOGY, 1988, 62 (11) :4113-4122
[2]   MUTATION OF A SITE OF TYROSINE PHOSPHORYLATION IN THE LYMPHOCYTE-SPECIFIC TYROSINE PROTEIN-KINASE, P56LCK, REVEALS ITS ONCOGENIC POTENTIAL IN FIBROBLASTS [J].
AMREIN, KE ;
SEFTON, BM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (12) :4247-4251
[3]   INTRONS INCREASE TRANSCRIPTIONAL EFFICIENCY IN TRANSGENIC MICE [J].
BRINSTER, RL ;
ALLEN, JM ;
BEHRINGER, RR ;
GELINAS, RE ;
PALMITER, RD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (03) :836-840
[4]   DISSECTION OF THYMOCYTE SIGNALING PATHWAYS BY INVIVO EXPRESSION OF PERTUSSIS TOXIN ADP-RIBOSYLTRANSFERASE [J].
CHAFFIN, KE ;
BEALS, CR ;
WILKIE, TM ;
FORBUSH, KA ;
SIMON, MI ;
PERLMUTTER, RM .
EMBO JOURNAL, 1990, 9 (12) :3821-3829
[5]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299
[6]  
Cooper J.A, 1990, PEPT PROT PHOSPH, P85
[7]   TYR527 IS PHOSPHORYLATED IN PP60C-SRC - IMPLICATIONS FOR REGULATION [J].
COOPER, JA ;
GOULD, KL ;
CARTWRIGHT, CA ;
HUNTER, T .
SCIENCE, 1986, 231 (4744) :1431-1434
[8]  
DIALYNAS DP, 1983, J IMMUNOL, V131, P2445
[9]   A PRACTICAL APPROACH FOR QUANTITATING SPECIFIC MESSENGER-RNAS BY SOLUTION HYBRIDIZATION [J].
DURNAM, DM ;
PALMITER, RD .
ANALYTICAL BIOCHEMISTRY, 1983, 131 (02) :385-393
[10]   SPECIFIC EXPRESSION OF A TYROSINE KINASE GENE, BLK, IN B-LYMPHOID CELLS [J].
DYMECKI, SM ;
NIEDERHUBER, JE ;
DESIDERIO, SV .
SCIENCE, 1990, 247 (4940) :332-336
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