INVOLVEMENT OF AUTOCRINE MECHANISM OF TRANSFORMING GROWTH-FACTOR-BETA IN THE FUNCTIONAL-DIFFERENTIATION OF PREGNANT MOUSE MAMMARY-GLAND

被引：8

作者：

YAMAMOTO, T ^{[1
]}

KOMURA, H ^{[1
]}

MORISHIGE, K ^{[1
]}

TADOKORO, C ^{[1
]}

SAKATA, M ^{[1
]}

KURACHI, H ^{[1
]}

MIYAKE, A ^{[1
]}

机构：

[1] OSAKA UNIV, SCH MED, DEPT OBSTET & GYNECOL, SUITA, OSAKA 565, JAPAN

来源：

EUROPEAN JOURNAL OF ENDOCRINOLOGY | 1994年 / 130卷 / 03期

关键词：

D O I：

10.1530/eje.0.1300302

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We studied the presence and possible role of the autocrine mechanism of transforming growth factor-beta (TGF-beta) in pregnant mouse mammary gland. Northern blot analysis revealed the expression of the TGF-beta(1) gene transcript at 2.5 kb in mammary epithelial cells isolated from virgin and mid-pregnant mice. The TGF-beta activity was higher in the conditioned medium from mid-pregnant mouse mammary explants than that from virgin explants by a bioassay system using mink lung epithelial cells. A binding study using [I-125]TGF-beta(1) as a ligand showed that pregnant mouse mammary epithelial cells possessed a single class of high-affinity TGF-beta(1) binding sites (K-d = 28.0 pmol/l, 1.2 x 10(4) sites per cell). These results suggested the presence of a TGF-beta autocrine mechanism in pregnant mouse mammary epithelial cells. Next, we examined the effect of TGF-beta(1) on the functional differentiation of pregnant mouse mammary gland. Transforming growth factor-beta(1) inhibited alpha-lactalbumin production in cultured mammary explants from mid-pregnant mice in a dose-dependent manner without inhibiting DNA synthesis. All these results suggest that TGF-beta may play a role in regulating the functional differentiation of mouse mammary glands during pregnancy.

引用

页码：302 / 307

页数：6

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