EFFECT OF SURFACTANTS ON CELLULOSE HYDROLYSIS

被引:212
作者
HELLE, SS
DUFF, SJB
COOPER, DG
机构
[1] UNIV BRITISH COLUMBIA, CTR PULP & PAPER, VANCOUVER V6T 1Z4, BC, CANADA
[2] UNIV BRITISH COLUMBIA, DEPT CHEM ENGN, VANCOUVER V6T 1Z4, BC, CANADA
[3] MCGILL UNIV, DEPT CHEM ENGN, MONTREAL H3A 2A7, QUEBEC, CANADA
关键词
SURFACTANT; CELLULOSE HYDROLYSIS; CELLULASE;
D O I
10.1002/bit.260420509
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The effect of surfactants on the heterogeneous enzymatic hydrolysis of Sigmacell 100 cellulose and of steam-exploded wood was studied. Certain biosurfactants (sophorolipid, rhamnolipid, bacitracin) and Tween 80 increased the rate of hydrolysis of Sigmacell 100, as measured by the amount of reducing sugar produced, by as much as seven times. The hydrolysis of steam-exploded wood was increased by 67% in the presence of sophorolipid. At the same time, sophorolipid was found to decrease the amount of enzyme adsorbed onto the cellulose at equilibrium. Sophorolipid had the greatest effect on cellulose hydrolysis when it was present from the beginning of the experiment and when the enzyme/cellulose ratio was low. (C) 1993 John Wiley & Sons, Inc.
引用
收藏
页码:611 / 617
页数:7
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