CLONING AND CHARACTERIZATION OF CDNAS THAT CODE FOR NA+-CHANNEL-BLOCKING TOXINS OF THE SCORPION CENTRUROIDES-NOXIUS HOFFMANN

被引：41

作者：

BECERRIL, B ^{[1
]}

VAZQUEZ, A ^{[1
]}

GARCIA, CI ^{[1
]}

CORONA, M ^{[1
]}

BOLIVAR, F ^{[1
]}

POSSANI, LD ^{[1
]}

机构：

[1] UNIV NACL AUTONOMA MEXICO, INST BIOTECNOL, AV UNIV 2001, APARTADO POSTAL 510-3, MORELOS 62271, MEXICO

来源：

GENE | 1993年 / 128卷 / 02期

关键词：

RECOMBINANT DNA; LAMBDA GT11 CDNA LIBRARY; SOUTHERN BLOT GENOMIC ANALYSIS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; TOXIN GENE EVOLUTION; INTRONS;

D O I：

10.1016/0378-1119(93)90559-L

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

With the purpose of studying the organization and characteristics of the genes that code for toxins present in the venom of the Mexican scorpion, Centruroides noxius Hoffmann (CnH), we prepared a lambdagt11 cDNA library from the venom glands. Using specific oligodeoxyribonucleotides (oligos) designed according to known amino acid (aa) sequences of CnH toxins (STox), we detected several positive clones, determined their nucleotide (nt) sequences and deduced their aa sequences. A comparative analysis of these sequences with previously reported STox revealed that CnH cDNAs code for a family of very similar STox. The cDNA coding for a known STox, II-10, was cloned. Additionally, three other complete (new) nt sequences were obtained for cDNAs encoding peptides similar to STox 1 from CnH or variants 2 and 3 from Centruroides sculpturatus Ewing. Southern blot genomic DNA analysis showed a minimum size of approximately 600 bp as EcoRI fragments for elements of this family. PCR amplifications of CnH genomic DNA and hybridization of PCR products with specific probes indicated that the genomic structural regions that code for these genes do not contain introns, or at least not large introns.

引用

页码：165 / 171

页数：7

共 29 条

[1] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].