CONSTRUCTION OF A TETRACYCLINE-INDUCIBLE PROMOTER IN SCHIZOSACCHAROMYCES-POMBE

被引：41

作者：

FARYAR, K ^{[1
]}

GATZ, C ^{[1
]}

机构：

[1] INST GENBIOL FORSCH BERLIN GMBH, IHNESTR 63, W-1000 BERLIN 33, GERMANY

来源：

CURRENT GENETICS | 1992年 / 21卷 / 4-5期

关键词：

CAMV-35S PROMOTER; TET REPRESSOR; TETRACYCLINE INDUCTION; SCHIZOSACCHAROMYCES-POMBE;

D O I：

10.1007/BF00351693

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have developed a tightly repressed Schizosaccharomyces pombe promoter which can be efficiently induced by Tetracycline. This promoter is a derivative of the plant viral cauliflower mosaic virus 35S promoter which normally functions as a strong constitutive promoter in S. pombe. Location of three binding sites for the Tn10-encoded Tet repressor in the vicinity of the TATA-box of the CaMV 35S promoter led to a tight repression of promoter activity in the presence of the Tet repressor protein. Up to a 400-fold induction was observed after addition of the inducer Tetracycline, which inactivates the operator-binding capacity of the repressor.

引用

页码：345 / 349

页数：5

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