IDENTIFICATION OF PRODUCTS FORMED DURING UV IRRADIATION OF TAMOXIFEN AND THEIR USE FOR FLUORESCENCE DETECTION IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：27

作者：

SALAMOUN, J

MACKA, M

NECHVATAL, M

MATOUSEK, M

KNESEL, L

机构：

[1] LACHEMA PEOPLES ENTERPRISE, RES INST PURE CHEM, CS-62133 BRNO, CZECHOSLOVAKIA

[2] CENT TESTING & CHECKING INST AGR, CS-65837 BRNO, CZECHOSLOVAKIA

[3] HYG LABS, REG HYG STN, CS-61800 BRNO, CZECHOSLOVAKIA

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 514卷 / 02期

关键词：

D O I：

10.1016/S0021-9673(01)89389-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

During the UV irradiation of tamoxifen, isomerization of the trans to the cis isomer takes place and consequently corresponding highly fluorescent phenanthrene derivatives are formed. Their formation can be used for the sensitive and selective detection of tamoxifen in high-performance liquid chromatography (HPLC). The structure of photoproducts was identified by >1H NMR spectroscopy, HPLC, gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry. Owing to the variety of products formed and the higher selectivity and fluorescence response, on-line postcolumn photocyclization is preferred to the precolumn mode. A chromatographic system for the separation of isomers and photoproducts is suggested. © 1990.

引用

页码：179 / 187

页数：9

共 14 条

[1] SEPARATION OF TAMOXIFEN GEOMETRIC ISOMERS AND METABOLITES BY BONDED-PHASE BETA-CYCLODEXTRIN CHROMATOGRAPHY [J].