ISOLATION OF PREADIPOCYTES FROM RAT ADIPOSE-TISSUE IN-VITRO

被引：0
作者：
KATOH, J
ISHIDA, Y
TANIGUCHI, H
机构：
[1] KOBE UNIV,SCH MED,HYOGO MED CTR ADULTS,DIV ENDOCRINOL & METAB,KOBE 650,JAPAN
[2] KOBE UNIV,SCH MED,DEPT INTERNAL MED 2,KOBE 650,JAPAN
来源：
FOLIA HISTOCHEMICA ET CYTOBIOLOGICA | 1994年 / 32卷 / 04期
关键词：
PREADIPOCYTES; PROLIFERATION; DIFFERENTIATION; CEILING CULTURE; GLYCEROPHOSPHATE DEHYDROGENASE;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Fibroblast-like cells, which would be differentiated from mature adipocytes in ''ceiling culture'', could be isolated in primary culture. These cells precipitated, were collected from the bottom of the flask of the ''ceiling culture'' and further proliferated to the confluence. They finally converted to morphologically and biochemically differentiated adipocytes. In these investigations we show how to isolate fibroblast-like cells and to identify these cells as ''preadipocytes''.
引用
收藏
页码：235 / 238
页数：4
相关论文
共 50 条
[21]   Rapid Isolation of Adipose Tissue-Derived Stem Cells by the Storage of Lipoaspirates [J].
Eom, Young Woo ;
Lee, Jong Eun ;
Yang, Mal Sook ;
Jang, In Keun ;
Kim, Hyo Eun ;
Lee, Doo Hoon ;
Kim, Young Jin ;
Park, Won Jin ;
Kong, Jee Hyun ;
Shim, Kwang Yong ;
Lee, Jong In ;
Kim, Hyun Soo .
YONSEI MEDICAL JOURNAL, 2011, 52 (06) :999-1007
[22]   Isolation of adipose tissue-derived stem cells [J].
Ozen, Asuman ;
Gul Sancak, Irem ;
Ceylan, Ahmet ;
Ozgenc, Ozge .
TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, 2016, 40 (02) :137-141
[23]   In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study [J].
Bearden, Robert N. ;
Huggins, Shannon S. ;
Cummings, Kevin J. ;
Smith, Roger ;
Gregory, Carl A. ;
Saunders, William B. .
STEM CELL RESEARCH & THERAPY, 2017, 8
[24]   In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study [J].
Robert N. Bearden ;
Shannon S. Huggins ;
Kevin J. Cummings ;
Roger Smith ;
Carl A. Gregory ;
William B. Saunders .
Stem Cell Research & Therapy, 8
[25]   Measuring committed preadipocytes in human adipose tissue from severely obese patients by using adipocyte fatty acid binding protein [J].
Tchoukalova, YD ;
Sarr, MG ;
Jensen, MD .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2004, 287 (05) :R1132-R1140
[26]   The Isolation of Pre-Adipocytes from Dairy Cow Adipose Tissue and the Development of Pre-Adipocytes into Mature Adipocytes [J].
Yin, Liheng ;
Qin, Xia ;
Deng, Qinghua ;
Zhang, Yuming ;
Lei, Lin ;
Gao, Wenwen ;
Peng, Zhicheng ;
Du, Xiliang ;
Liu, Guowen ;
Li, Xinwei .
PAKISTAN VETERINARY JOURNAL, 2015, 35 (03) :283-288
[27]   Isolation of adipose tissue derived regenerative cells from human subcutaneous tissue with or without the use of an enzymatic reagent [J].
Winnier, Glenn E. ;
Valenzuela, Nick ;
Peters-Hall, Jennifer ;
Kellner, Joshua ;
Alt, Christopher ;
Alt, Eckhard U. .
PLOS ONE, 2019, 14 (09)
[28]   Differentiation of rat adipose tissue-derived stem cells into Schwann-like cells in vitro [J].
Jiang, Li ;
Zhu, Jia-Kai ;
Liu, Xiao-Lin ;
Xiang, Peng ;
Hu, Jun ;
Yu, Wei-Hua .
NEUROREPORT, 2008, 19 (10) :1015-1019
[29]   Development of novel human in vitro vascularized adipose tissue model with functional macrophages [J].
Huttala, Outi ;
Sarkanen, Jertta-Riina ;
Mannerstrom, Marika ;
Toimela, Tarja ;
Heinonen, Tuula ;
Ylikomi, Timo .
CYTOTECHNOLOGY, 2020, 72 (05) :665-683
[30]   Repurposed Drugs to Enhance the Therapeutic Potential of Oligodendrocyte Precursor Cells Derived from Adult Rat Adipose Tissue [J].
Pascual-Guerra, J. ;
Torres-Rico, M. ;
Marin-Rodriguez, B. ;
Arasmou-Idrovo, M. S. ;
Garcia, A. G. ;
Rodriguez-Navarro, J. A. ;
Paino, C. L. .
CELLS, 2025, 14 (07)
12345