ISOLATION OF PREADIPOCYTES FROM RAT ADIPOSE-TISSUE IN-VITRO

被引：0

作者：

KATOH, J

ISHIDA, Y

TANIGUCHI, H

机构：

[1] KOBE UNIV,SCH MED,HYOGO MED CTR ADULTS,DIV ENDOCRINOL & METAB,KOBE 650,JAPAN

[2] KOBE UNIV,SCH MED,DEPT INTERNAL MED 2,KOBE 650,JAPAN

来源：

FOLIA HISTOCHEMICA ET CYTOBIOLOGICA | 1994年 / 32卷 / 04期

关键词：

PREADIPOCYTES; PROLIFERATION; DIFFERENTIATION; CEILING CULTURE; GLYCEROPHOSPHATE DEHYDROGENASE;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fibroblast-like cells, which would be differentiated from mature adipocytes in ''ceiling culture'', could be isolated in primary culture. These cells precipitated, were collected from the bottom of the flask of the ''ceiling culture'' and further proliferated to the confluence. They finally converted to morphologically and biochemically differentiated adipocytes. In these investigations we show how to isolate fibroblast-like cells and to identify these cells as ''preadipocytes''.

引用

页码：235 / 238

页数：4

共 12 条

[1] COUPLING OF GROWTH ARREST AND EXPRESSION OF EARLY MARKERS DURING ADIPOSE CONVERSION OF PREADIPOCYTE CELL-LINES [J].