DEVELOPMENT OF A BISPECIFIC MONOCLONAL-ANTIBODY FOR USE IN MOLECULAR HYBRIDIZATION

被引：12
作者：
AURIOL, J
GUESDON, JL
MAZIE, JC
NATO, F
机构：
[1] INST PASTEUR,PREDEV SONDES LAB,F-75724 PARIS 15,FRANCE
[2] INST PASTEUR,HYBRIDOLAB,PARIS,FRANCE
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1994年 / 169卷 / 01期
关键词：
BISPECIFIC ANTIBODY; HYBRID HYBRIDOMA; MOLECULAR HYBRIDIZATION; NONRADIOACTIVE PROBE;
D O I：
10.1016/0022-1759(94)90131-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A mouse hybrid hybridoma (tetradoma) was prepared by fusing hybridomas producing monoclonal antibody to acetyl-aminofluorene with hybridomas producing antibody against calf intestine alkaline phosphatase. The tetradoma line established secreted immunoglobulin manifesting parental and bispecific binding characteristics. Bispecific monoclonal antibody was purified and used for a one-step immunodetection assay of non-radioactive DNA and RNA probes. The immunoassay developed was able to detect 5 pg DNA within 2 h and gave low background noise.
引用
收藏
页码：123 / 133
页数：11
相关论文
共 32 条
  • [1] ANTIGEN-BINDING PROPERTIES OF HIGHLY PURIFIED BISPECIFIC ANTIBODIES
    ALLARD, WJ
    MORAN, CA
    NAGEL, E
    COLLINS, G
    LARGEN, MT
    [J]. MOLECULAR IMMUNOLOGY, 1992, 29 (10) : 1219 - 1227
  • [2] ANDREW SM, 1990, BISPECIFIC ANTIBODIE, P197
  • [3] CHARACTERIZATION OF XANTHOMONAS-CAMPESTRIS PATHOVARS BY RIBOSOMAL-RNA GENE RESTRICTION PATTERNS
    BERTHIER, Y
    VERDIER, V
    GUESDON, JL
    CHEVRIER, D
    DENIS, JB
    DECOUX, G
    LEMATTRE, M
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (03) : 851 - 859
  • [4] A SIMPLE METABOLIC SYSTEM FOR SELECTION OF HYBRID HYBRIDOMAS (TETRADOMAS) PRODUCING BISPECIFIC MONOCLONAL-ANTIBODIES
    CHERVONSKY, AV
    FAERMAN, AI
    EVDONINA, LV
    JAZOVA, AK
    KAZAROV, AR
    GUSSEV, AI
    [J]. MOLECULAR IMMUNOLOGY, 1988, 25 (09) : 913 - 915
  • [5] USEFULNESS OF THE ID32 STAPH SYSTEM AND A METHOD BASED ON RIBOSOMAL-RNA GENE RESTRICTION SITE POLYMORPHISM ANALYSIS FOR SPECIES AND SUBSPECIES IDENTIFICATION OF STAPHYLOCOCCAL CLINICAL ISOLATES
    CHESNEAU, O
    AUBERT, S
    MORVAN, A
    GUESDON, JL
    ELSOLH, N
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (09) : 2346 - 2352
  • [6] CLARK MR, 1987, J NATL CANCER I, V79, P1393
  • [7] DELAU WBM, 1990, J IMMUNOL, V146, P906
  • [8] MEASUREMENTS OF THE TRUE AFFINITY CONSTANT IN SOLUTION OF ANTIGEN-ANTIBODY COMPLEXES BY ENZYME-LINKED IMMUNOSORBENT-ASSAY
    FRIGUET, B
    CHAFFOTTE, AF
    DJAVADIOHANIANCE, L
    GOLDBERG, ME
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1985, 77 (02) : 305 - 319
  • [9] GLENNIE MJ, 1987, J IMMUNOL, V139, P2367
  • [10] ACETYLAMINOFLUORENE-LABELED RIBOSOMAL-RNA FOR USE IN MOLECULAR EPIDEMIOLOGY AND TAXONOMY
    GRIMONT, F
    CHEVRIER, D
    GRIMONT, PAD
    LEFEVRE, M
    GUESDON, JL
    [J]. RESEARCH IN MICROBIOLOGY, 1989, 140 (07) : 447 - 454
1234