DEVELOPMENT OF A BISPECIFIC MONOCLONAL-ANTIBODY FOR USE IN MOLECULAR HYBRIDIZATION

被引：12
作者：
AURIOL, J
GUESDON, JL
MAZIE, JC
NATO, F
机构：
[1] INST PASTEUR,PREDEV SONDES LAB,F-75724 PARIS 15,FRANCE
[2] INST PASTEUR,HYBRIDOLAB,PARIS,FRANCE
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1994年 / 169卷 / 01期
关键词：
BISPECIFIC ANTIBODY; HYBRID HYBRIDOMA; MOLECULAR HYBRIDIZATION; NONRADIOACTIVE PROBE;
D O I：
10.1016/0022-1759(94)90131-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A mouse hybrid hybridoma (tetradoma) was prepared by fusing hybridomas producing monoclonal antibody to acetyl-aminofluorene with hybridomas producing antibody against calf intestine alkaline phosphatase. The tetradoma line established secreted immunoglobulin manifesting parental and bispecific binding characteristics. Bispecific monoclonal antibody was purified and used for a one-step immunodetection assay of non-radioactive DNA and RNA probes. The immunoassay developed was able to detect 5 pg DNA within 2 h and gave low background noise.
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页码：123 / 133
页数：11
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