Viral diagnosis in Indian livestock using customized microarray chips

被引：2

作者：

Yadav, Brijesh S. ^{[1
]}

Pokhriyal, Mayank ^{[1
]}

Ratta, Barkha ^{[1
]}

Kumar, Ajay ^{[1
]}

Saxena, Meeta ^{[1
]}

Sharma, Bhaskar ^{[1
]}

机构：

[1] Indian Vet Res Inst, Div Biochem, Izatnagar 243122, Uttar Pradesh, India

来源：

BIOINFORMATION | 2015年 / 11卷 / 11期

关键词：

DNA microarray; viral diagnosis; livestock; RT-PCR (reverse transcriptase polymerase chain reaction);

D O I：

10.6026/97320630011489

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Viral diagnosis in Indian livestock using customized microarray chips is gaining momentum in recent years. Hence, it is possible to design customized microarray chip for viruses infecting livestock in India. Customized microarray chips identified Bovine herpes virus-1 (BHV-1), Canine Adeno Virus-1 (CAV-1), and Canine Parvo Virus-2 (CPV-2) in clinical samples. Microarray identified specific probes were further confirmed using RT-PCR in all clinical and known samples. Therefore, the application of microarray chips during viral disease outbreaks in Indian livestock is possible where conventional methods are unsuitable. It should be noted that customized application requires a detailed cost efficiency calculation.

引用

页码：489 / 492

页数：4

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