A STAPHYLOCOCCUS-AUREUS-SPECIFIC OLIGONUCLEOTIDE PROBE DERIVED FROM 16S RIBOSOMAL-RNA GENE-SEQUENCES

被引：17

作者：

BENTLEY, RW ^{[1
]}

HARLAND, NM ^{[1
]}

LEIGH, JA ^{[1
]}

COLLINS, MD ^{[1
]}

机构：

[1] INST FOOD RES, READING RG6 2EF, ENGLAND

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1993年 / 16卷 / 04期

关键词：

D O I：

10.1111/j.1472-765X.1993.tb01397.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genes encoding 16S rRNA were sequenced from 16 species of Staphylococcus. Sequence analysis highlighted a potential Staph. aureus-specific region and a complementary oligonucleotide probe was synthesized and its specificity tested. Northern blotting indicated molecular specificity, and dot blots to RNA from Staph. aureus, Staph. capitis, Staph. caprae, Staph. carnosus, Staph. caseolyticus, Staph. cohnii, Staph. epidermidis, Staph. gallinarum, Staph. haemolyticus, Staph. hominis, Staph. hyicus, Staph. saprophyticus, Staph. sciuri, Staph. simulans, Staph. warneri and Staph. xylosus indicated species-specificity.

引用

页码：203 / 206

页数：4

共 13 条

[1] DEOXYNUCLEOSIDE PHOSPHORAMIDITES - A NEW CLASS OF KEY INTERMEDIATES FOR DEOXYPOLYNUCLEOTIDE SYNTHESIS [J].