ISOLATION OF ACIDIC ACROSIN ISOINHIBITORS (BUSI-IA, BUSI-IB1 AND BUSI-IB2) FROM BULL SEMINAL PLASMA

被引：18

作者：

CECHOVA, D

JONAKOVA, V

HAVRANOVA, M

SEDLAKOVA, E

MACH, O

机构：

[1] Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague

来源：

HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE | 1979年 / 360卷 / 12期

关键词：

acrosin inhibitor; fertilization; isoinhibitors; Proteinase inhibitors; seminal plasma;

D O I：

10.1515/bchm2.1979.360.2.1759

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three natural proteinase isoinhibitors with low isoelectric points BUSI [bull seminal plasma inhibitor] I A (pI = 3.9), BUSI I B1 (pI= 3.4) and BUSI I B2 (pI = 3.7) were isolated from bull seminal plasma by gel filtration on Sephadex G-50 and ion exchange chromatography on DEAE-Sephadex and SE-Sephadex. Isoinhibitors B1 and B2 have identical amino acid composition. Isoinhibitor A contains 6 amino acid residues less than isoinhibitors B1 and B2. Since sugars were detected in the isoinhibitors, heterogeneity may also be due to the sugar component. The isoinhibitors show the same inhibitory properties; all of them inhibit acrosin, trypsin (EC 3.4.21.4) and chymotrypsin (EC 3.4.21.1). Glandular kallikrein is also inhibited, but to a very low extent only. The MW (.apprx. 8900) was determined by gel filtration.

引用

页码：1759 / 1766

页数：8

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