ISOLATION OF ACIDIC ACROSIN ISOINHIBITORS (BUSI-IA, BUSI-IB1 AND BUSI-IB2) FROM BULL SEMINAL PLASMA

被引：18
作者：
CECHOVA, D
JONAKOVA, V
HAVRANOVA, M
SEDLAKOVA, E
MACH, O
机构：
[1] Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague
来源：
HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE | 1979年 / 360卷 / 12期
关键词：
acrosin inhibitor; fertilization; isoinhibitors; Proteinase inhibitors; seminal plasma;
D O I：
10.1515/bchm2.1979.360.2.1759
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Three natural proteinase isoinhibitors with low isoelectric points BUSI [bull seminal plasma inhibitor] I A (pI = 3.9), BUSI I B1 (pI= 3.4) and BUSI I B2 (pI = 3.7) were isolated from bull seminal plasma by gel filtration on Sephadex G-50 and ion exchange chromatography on DEAE-Sephadex and SE-Sephadex. Isoinhibitors B1 and B2 have identical amino acid composition. Isoinhibitor A contains 6 amino acid residues less than isoinhibitors B1 and B2. Since sugars were detected in the isoinhibitors, heterogeneity may also be due to the sugar component. The isoinhibitors show the same inhibitory properties; all of them inhibit acrosin, trypsin (EC 3.4.21.4) and chymotrypsin (EC 3.4.21.1). Glandular kallikrein is also inhibited, but to a very low extent only. The MW (.apprx. 8900) was determined by gel filtration.
引用
收藏
页码：1759 / 1766
页数：8
相关论文
共 16 条
  • [1] CECHOVA D, 1979, H-S Z PHYSIOL CHEM, V360, P1753, DOI 10.1515/bchm2.1979.360.2.1753
  • [2] CHARACTERIZATION OF PROTEINASE-INHIBITORS FROM BULL SEMINAL PLASMA AND SPERMATOZOA
    CECHOVA, D
    FRITZ, H
    [J]. HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1976, 357 (03): : 401 - 408
  • [3] CECHOVA D, 1978, 12TH FEBS M DRESD
  • [4] A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS
    DUBOIS, M
    GILLES, K
    HAMILTON, JK
    REBERS, PA
    SMITH, F
    [J]. NATURE, 1951, 168 (4265) : 167 - 167
  • [5] FINK E, 1971, P INT RES C PROTEINA, P225
  • [6] NATURALLY OCCURRING LOW-MOLECULAR WEIGHT INHIBITORS OF NEUTRAL PROTEINASES FROM PMN-GRANULOCYTES AND OF KALLIKREINS
    FRITZ, H
    SCHIESSLER, H
    GEIGER, R
    OHLSSON, K
    HOCHSTRASSER, K
    [J]. AGENTS AND ACTIONS, 1978, 8 (1-2): : 57 - 64
  • [7] Fritz H., 1974, METHOD ENZYMAT AN, P1064, DOI 10.1016/B978-0-12-091302-2.X5001-4
  • [8] JONAKOVA V, 1979, FEBS SPECIAL M ENZYM
  • [9] HIGH RECOVERY OF TRYPTOPHAN FROM ACID HYDROLYSATES OF PROTEINS
    MATSUBARA, H
    SASAKI, RM
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1969, 35 (02) : 175 - +
  • [10] SCHIESSLER H, 1974, 5 BAY S PROT INH, P147
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