PERTUSSIS TOXIN DOES NOT INHIBIT ALPHA-1-ADRENERGIC POTENTIATION OF BETA-ADRENERGIC STIMULATION OF CYCLIC-AMP ACCUMULATION IN RAT PINEALOCYTES

被引：3
作者：
SUGDEN, D
机构：
[1] Biomedical Sciences Division, King's College London, London, W8 7AH, Campden Hill Road, Kensington
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 02期
关键词：
D O I：
10.1016/0006-291X(90)92402-L
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The hypothesis that Gi might be involved in the α1-adrenergic, protein kinase C (PKC)-mediated amplification of β-adrenergic cyclic AMP stimulation in rat pinealocytes was investigated. Treatment of pinealocytes with a high concentration of pertussis toxin (500 ng/ml, 18 h) almost completely (approximately 95%) inactivated two cell membrane G-proteins (kDa 40.7 and 39.8) judged by back ADP-ribosylation of pinealocyte membrane proteins. However, this treatment failed to inhibit either the β-adrenergic (isoprepaline, ISO 10-6 M), α1-plus β-adrenergic (noradrenaline, NA 10-5 M) or β -adrenergic plus 12-O-tetradecanoylphorbol 13-acetate (TPA 10-7 M) induced stimulation of cyclic AMP or cyclic GMP. These results suggest that α1-adrenergic potentiation of β-adrenergic stimulation of cyclic AMP and cyclic GMP does not involve a pertussis toxin-sensitive G-protein. © 1990.
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页码：871 / 877
页数：7
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