BEHAVIOR OF LIVING PRIMORDIAL GERM-CELLS OF LIVESTOCK INVITRO

被引：17
作者：
LEICHTHAMMER, F [1 ]
BAUNACK, E [1 ]
BREM, G [1 ]
机构：
[1] MED HSCH HANOVER, CENT ANIM LAB, W-3000 Hannover 61, GERMANY
来源：
THERIOGENOLOGY | 1990年 / 33卷 / 06期
关键词：
in vitro culture; primordial germ cells; sex differentiation;
D O I：
10.1016/0093-691X(90)90040-Z
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Primordial germ cells of the livestock species cow, pig and rabbit were obtained from the fetuses of animals slaughtered at varying periods of gestation and cultivated in vitro. The detection of alkaline phosphatase was used as a specific marker. The survival rate was determined at defined time intervals, and changes in morphological characteristics were observed. The survival rate of the cells in vitro was low, especially when cultured for more than 24 h. The results indicate that changes in the cellular morphology are analogous to those that occur in vivo, but that these changes are not as pronounced in livestock animals as in the mouse. © 1990.
引用
收藏
页码：1221 / 1230
页数：10
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