A NEW, HIGHLY SENSITIVE METHOD FOR THE DETECTION AND QUANTIFICATION OF PENICILLIN-BINDING PROTEINS

被引：28
作者：
GALLENI, M
LAKAYE, B
LEPAGE, S
JAMIN, M
THAMM, I
JORIS, B
FRERE, JM
机构：
[1] UNIV LIEGE,INST CHIM,ENZYMOL LAB,B6,B-4000 SART,BELGIUM
[2] UNIV LIEGE,INST CHIM,CTR INGN PROT,B-4000 SART,BELGIUM
来源：
BIOCHEMICAL JOURNAL | 1993年 / 291卷
关键词：
D O I：
10.1042/bj2910019
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the rapid detection of 0.2 pmol with the naked eye and 2 fmol with the help of an A.L.F. automatic DNA sequencer. Direct labelling can also be performed on whole bacterial cells.
引用
收藏
页码：19 / 21
页数：3
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