A NEW, HIGHLY SENSITIVE METHOD FOR THE DETECTION AND QUANTIFICATION OF PENICILLIN-BINDING PROTEINS

被引：28
作者：
GALLENI, M
LAKAYE, B
LEPAGE, S
JAMIN, M
THAMM, I
JORIS, B
FRERE, JM
机构：
[1] UNIV LIEGE,INST CHIM,ENZYMOL LAB,B6,B-4000 SART,BELGIUM
[2] UNIV LIEGE,INST CHIM,CTR INGN PROT,B-4000 SART,BELGIUM
来源：
BIOCHEMICAL JOURNAL | 1993年 / 291卷
关键词：
D O I：
10.1042/bj2910019
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the rapid detection of 0.2 pmol with the naked eye and 2 fmol with the help of an A.L.F. automatic DNA sequencer. Direct labelling can also be performed on whole bacterial cells.
引用
收藏
页码：19 / 21
页数：3
相关论文
共 50 条
[31]   Detection of penicillin-binding proteins in Bacillus cereus by using biotinylated β-lactams [J].
Miyamoto, T ;
Sukimoto, K ;
Abu Sayed, M ;
Kim, SI ;
Honjoh, K ;
Hatano, S .
JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, 2000, 44 (3-4) :299-307
[32]   PENICILLIN-BINDING PROTEINS IN BACTEROIDES-FRAGILIS [J].
GEORGOPAPADAKOU, NH ;
SMITH, SA ;
SYKES, RB .
JOURNAL OF ANTIBIOTICS, 1983, 36 (07) :907-910
[33]   PENICILLIN-BINDING PROTEINS IN THE MEMBRANES OF STREPTOMYCES SP [J].
DUSART, J ;
LEYHBOUILLE, M ;
NGUYENDISTECHE, M ;
GHUYSEN, JM ;
REYNOLDS, PE .
ARCHIVES INTERNATIONALES DE PHYSIOLOGIE DE BIOCHIMIE ET DE BIOPHYSIQUE, 1980, 88 (01) :B27-B29
[34]   USE OF PENICILLIN-BINDING PROTEINS FOR THE IDENTIFICATION OF ENTEROCOCCI [J].
WILLIAMSON, R ;
GUTMANN, L ;
HORAUD, T ;
DELBOS, F ;
ACAR, JF .
JOURNAL OF GENERAL MICROBIOLOGY, 1986, 132 :1929-1937
[35]   PENICILLIN-BINDING PROTEINS OF STREPTOCOCCUS-PNEUMONIAE [J].
CHALKLEY, LJ ;
KOORNHOF, HJ .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1988, 22 (06) :791-800
[36]   PENICILLIN-BINDING PROTEINS OF BACTEROIDES-FRAGILIS [J].
BOTTA, GA ;
PRIVITERA, G ;
MENOZZI, MG .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1984, 14 (02) :197-199
[37]   β-lactam resistance in Streptococcus pneumoniae:: penicillin-binding proteins and non-penicillin-binding proteins [J].
Hakenbeck, R ;
Grebe, T ;
Zähner, D ;
Stock, JB .
MOLECULAR MICROBIOLOGY, 1999, 33 (04) :673-678
[38]   PENICILLIN-BINDING PROTEINS OF NUTRITIONALLY VARIANT STREPTOCOCCI [J].
GUTMANN, L ;
BOUVET, A ;
ACAR, JF .
FEMS MICROBIOLOGY LETTERS, 1982, 14 (01) :11-14
[39]   PENICILLIN-BINDING PROTEINS IN SELENOMONAS-RUMINANTIUM [J].
KAMIO, Y ;
TERAWAKI, Y .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1981, 45 (04) :993-995
[40]   PENICILLIN-BINDING PROTEINS OF HEMOPHILUS-DUCREYI [J].
LEE, BC ;
BRYAN, LE .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1989, 33 (06) :983-986
12345