A NEW, HIGHLY SENSITIVE METHOD FOR THE DETECTION AND QUANTIFICATION OF PENICILLIN-BINDING PROTEINS

被引：28
作者：
GALLENI, M
LAKAYE, B
LEPAGE, S
JAMIN, M
THAMM, I
JORIS, B
FRERE, JM
机构：
[1] UNIV LIEGE,INST CHIM,ENZYMOL LAB,B6,B-4000 SART,BELGIUM
[2] UNIV LIEGE,INST CHIM,CTR INGN PROT,B-4000 SART,BELGIUM
来源：
BIOCHEMICAL JOURNAL | 1993年 / 291卷
关键词：
D O I：
10.1042/bj2910019
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the rapid detection of 0.2 pmol with the naked eye and 2 fmol with the help of an A.L.F. automatic DNA sequencer. Direct labelling can also be performed on whole bacterial cells.
引用
收藏
页码：19 / 21
页数：3
相关论文
共 50 条
[21]   THE LACK OF PENICILLIN-BINDING PROTEINS IN MYCOPLASMAS [J].
REYNOLDS, PE ;
ARCHER, DB .
REVIEWS OF INFECTIOUS DISEASES, 1982, 4 :S265-S265
[22]   Penicillin-binding proteins in Leptospira interrogans [J].
Brenot, A ;
Trott, D ;
Saint Girons, I ;
Zuerner, R .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (03) :870-877
[23]   PROPERTIES OF THE PENICILLIN-BINDING PROTEINS OF CAULOBACTER [J].
KOYASU, S ;
FUKUDA, A ;
OKADA, Y .
JOURNAL OF GENERAL MICROBIOLOGY, 1981, 126 (SEP) :111-121
[24]   AFFINITY OF CEFOPERAZONE FOR PENICILLIN-BINDING PROTEINS [J].
MATSUBARA, N ;
MINAMI, S ;
MATSUHASHI, M ;
TAKAOKA, M ;
MITSUHASHI, S .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1980, 18 (01) :195-199
[25]   PENICILLIN-BINDING PROTEINS IN PROTEUS SPECIES [J].
OHYA, S ;
YAMAZAKI, M ;
SUGAWARA, S ;
MATSUHASHI, M .
JOURNAL OF BACTERIOLOGY, 1979, 137 (01) :474-479
[26]   PENICILLIN-BINDING PROTEINS IN THEORY AND PRACTICE [J].
CURTIS, NAC .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1981, 8 (02) :85-87
[27]   Penicillin-binding proteins in Fusobacterium species [J].
Tuner, K ;
Wexler, HM ;
Reeves, D ;
Finegold, SM .
ANAEROBE, 1996, 2 (03) :155-162
[28]   Design of new antimicrobials directed against penicillin-binding proteins [J].
Nicola, G ;
Davies, C .
BIOPHYSICAL JOURNAL, 2005, 88 (01) :333A-334A
[29]   New Noncovalent Inhibitors of Penicillin-Binding Proteins from Penicillin-Resistant Bacteria [J].
Turk, Samo ;
Verlaine, Olivier ;
Gerards, Thomas ;
Zivec, Matej ;
Humljan, Jan ;
Sosic, Izidor ;
Amoroso, Ana ;
Zervosen, Astrid ;
Luxen, Andre ;
Joris, Bernard ;
Gobec, Stanislav .
PLOS ONE, 2011, 6 (05)
[30]   DIGOXIGENIN-AMPICILLIN CONJUGATE FOR DETECTION OF PENICILLIN-BINDING PROTEINS BY CHEMILUMINESCENCE [J].
WEIGEL, LM ;
BELISLE, JT ;
RADOLF, JD ;
NORGARD, MV .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1994, 38 (02) :330-336
12345